MAD2 antibody | G01/1A11

Mouse anti MAD2

Product Type
PrecisionAb Monoclonal
Clone
G01/1A11
Isotype
IgG1
Format
Purified
Specificity
MAD2

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VMA00647
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WB 100 µl loader
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Mouse anti Human MAD2 antibody recognizes MAD2, also known as mitotic arrest deficient 2-like protein 1 (MAD2L1) or mitotic spindle assembly checkpoint protein MAD2A.

MAD2 is a ubiquitously-expressed protein which plays an essential role in the cell cycle, monitoring correct chromosomal alignment at the metaphase plate before mitosis. MAD2 is often overexpressed in tumors and associated with increased risk of all-cause mortality and cancer recurrence. Functionally, due to its role in the cell cycle, MAD2 may be important for driving the chromosome instability that initiates tumorigenesis. Beyond the spindle assembly checkpoint, MAD2 interacts with various proteins including insulin receptor, estrogen receptor beta, and tumor necrosis factor alpha convertase (Marks et al. 2017).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti MAD2 antibody detects a band of approximately 23 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human MAD2 (aa 1-205)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q13257
Entrez Gene
MAD2L1
GO Terms
GO:0000236 mitotic prometaphase
GO:0000777 condensed chromosome kinetochore
GO:0005829 cytosol
GO:0005634 nucleus
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0042803 protein homodimerization activity
GO:0043066 negative regulation of apoptosis
GO:0048471 perinuclear region of cytoplasm
GO:0051301 cell division
GO:0060564 negative regulation of mitotic anaphase-promoting complex activity
GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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