MAD2 antibody | G01/1A11
Mouse anti Human MAD2 antibody recognizes MAD2, also known as mitotic arrest deficient 2-like protein 1 (MAD2L1) or mitotic spindle assembly checkpoint protein MAD2A.
MAD2 is a ubiquitously-expressed protein which plays an essential role in the cell cycle, monitoring correct chromosomal alignment at the metaphase plate before mitosis. MAD2 is often overexpressed in tumors and associated with increased risk of all-cause mortality and cancer recurrence. Functionally, due to its role in the cell cycle, MAD2 may be important for driving the chromosome instability that initiates tumorigenesis. Beyond the spindle assembly checkpoint, MAD2 interacts with various proteins including insulin receptor, estrogen receptor beta, and tumor necrosis factor alpha convertase (Marks et al. 2017).
MAD2 is a ubiquitously-expressed protein which plays an essential role in the cell cycle, monitoring correct chromosomal alignment at the metaphase plate before mitosis. MAD2 is often overexpressed in tumors and associated with increased risk of all-cause mortality and cancer recurrence. Functionally, due to its role in the cell cycle, MAD2 may be important for driving the chromosome instability that initiates tumorigenesis. Beyond the spindle assembly checkpoint, MAD2 interacts with various proteins including insulin receptor, estrogen receptor beta, and tumor necrosis factor alpha convertase (Marks et al. 2017).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti MAD2 antibody detects a band of approximately 23 kDa in K562 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human MAD2 (aa 1-205)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13257
- Entrez Gene
- MAD2L1
- GO Terms
- GO:0000236 mitotic prometaphase
- GO:0000777 condensed chromosome kinetochore
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0042803 protein homodimerization activity
- GO:0043066 negative regulation of apoptosis
- GO:0048471 perinuclear region of cytoplasm
- GO:0051301 cell division
- GO:0060564 negative regulation of mitotic anaphase-promoting complex activity
- GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of MAD2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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