Macrophage antibody | PM-2K

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Mouse anti Human Macrophage (Tissue and Proliferative Disorders)

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Mouse anti Human macrophages, clone PM-2K recognizes most tissue macrophages in thymus, spleen, lymph nodes and tonsil. It is positive on Kupffer cells (liver), alveolar macrophages and macrophages in the interstitial tissue of the kidney, pancreas and other organs.

Mouse anti Human macrophages, clone PM-2K does not react with macrophage scavenger receptors AI and AII.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilized
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Kathon™
0.5% Bovine Serum Albumin
Cultured human peritoneal macrophages.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml after reconstitution

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

For research purposes only

Applications of Macrophage antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 1/200 1/800
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Western Blotting
5870-8008 recognizes an antigen of 150 kDa on macrophage cell membranes.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunohistology - Paraffin

Product Specific References

Source Reference

  1. Takeya, M. et al. (1991) A new monoclonal antibody, PM-2K, specifically recognizes tissue macrophages but not blood monocytes.
    J Pathol. 163 (4): 315-21.

References for Macrophage antibody

  1. Frangogiannis, N.G. et al. (2002) Evidence for an active inflammatory process in the hibernating human myocardium.
    Am J Pathol. 160: 1425-33.
  2. Kume, S. et al. (1995) Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta with a novel specific monoclonal antibody.
    Am J Pathol. 147: 654-67.
  3. Frangogiannis, N.G. et al. (2003) MCSF expression is induced in healing myocardial infarcts and may regulate monocyte and endothelial cell phenotype.
    Am J Physiol Heart Circ Physiol. 285: H483-92.
  4. Somasundaram, P. et al. (2005) Mast cell tryptase may modulate endothelial cell phenotype in healing myocardial infarcts.
    J Pathol. 205: 102-11.
  5. Zeng, L. et al. (1996) Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
    J Histochem Cytochem. 44: 845-53.
  6. Horiuchi, S. et al. (1996) Extra- and intracellular localization of advanced glycation end-products in human atherosclerotic lesions.
    Nephrol Dial Transplant. 11 Suppl 5: 81-6.
  7. Dobaczewski, M. et al. (2006) Extracellular matrix remodeling in canine and mouse myocardial infarcts.
    Cell Tissue Res. 324: 475-88.
  8. Dewald, O. et al. (2004) Of mice and dogs: species-specific differences in the inflammatory response following myocardial infarction
    Am J Pathol. 164: 665-77.
  9. Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
    PLoS One. 4: e7475.
  10. Ribeiro Dos Santos, P. et al. (2011) Rapid dissemination of SIV follows multisite entry after rectal inoculation.
    PLoS One. 6 (5): e19493.
  11. Kamihata, H. et al. (2001) Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines.
    Circulation. 104 (9): 1046-52.
  12. Riddell, M.R. et al. (2012) The characterization of fibrocyte-like cells: a novel fibroblastic cell of the placenta.
    Placenta. 33 (3): 143-50.
  13. Hung, C.H. et al. (2018) Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
    Sci Rep. 8 (1): 919.
  14. Hung, C.H. et al. (2018) Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer.
    BMC Cancer. 18 (1): 366.
  15. Hsieh, S.W. et al. (2020) M2b macrophage subset decrement as an indicator of cognitive function in Alzheimer's disease.
    Psychiatry Clin Neurosci. 74 (7): 383-91.
  16. Kuo, C. et al. (2021) Altered Pattern of Macrophage Polarization as a Biomarker for Severity of Childhood Asthma
    Journal of Inflammation Research. Volume 14: 6011-6023.

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