Macrophage antibody | PM-2K

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Mouse anti Human Macrophage (Tissue and Proliferative Disorders)

Product Type
Monoclonal Antibody
Clone
PM-2K
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5870-8008 C* WB 0.1 mg
Mouse anti Human macrophages, clone PM-2K recognizes most tissue macrophages in thymus, spleen, lymph nodes and tonsil. It is positive on Kupffer cells (liver), alveolar macrophages and macrophages in the interstitial tissue of the kidney, pancreas and other organs.

Mouse anti Human macrophages, clone PM-2K does not react with macrophage scavenger receptors AI and AII.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Hamster
Guinea Pig
Pig
Monkey
Horse
Cat
Rat
Bovine
Goat
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Kathon™
0.5% Bovine Serum Albumin
Immunogen
Cultured human peritoneal macrophages.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml after reconstitution

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of Macrophage antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 1/200 1/800
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil
Western Blotting
5870-8008 recognizes an antigen of 150 kDa on macrophage cell membranes.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

Source Reference

  1. Takeya, M. et al. (1991) A new monoclonal antibody, PM-2K, specifically recognizes tissue macrophages but not blood monocytes.
    J Pathol. 163 (4): 315-21.

References for Macrophage antibody

  1. Frangogiannis, N.G. et al. (2002) Evidence for an active inflammatory process in the hibernating human myocardium.
    Am J Pathol. 160: 1425-33.
  2. Kume, S. et al. (1995) Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta with a novel specific monoclonal antibody.
    Am J Pathol. 147: 654-67.
  3. Frangogiannis, N.G. et al. (2003) MCSF expression is induced in healing myocardial infarcts and may regulate monocyte and endothelial cell phenotype.
    Am J Physiol Heart Circ Physiol. 285: H483-92.
  4. Somasundaram, P. et al. (2005) Mast cell tryptase may modulate endothelial cell phenotype in healing myocardial infarcts.
    J Pathol. 205: 102-11.
  5. Zeng, L. et al. (1996) Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
    J Histochem Cytochem. 44: 845-53.
  6. Horiuchi, S. et al. (1996) Extra- and intracellular localization of advanced glycation end-products in human atherosclerotic lesions.
    Nephrol Dial Transplant. 11 Suppl 5: 81-6.
  7. Dobaczewski, M. et al. (2006) Extracellular matrix remodeling in canine and mouse myocardial infarcts.
    Cell Tissue Res. 324: 475-88.
  8. Dewald, O. et al. (2004) Of mice and dogs: species-specific differences in the inflammatory response following myocardial infarction
    Am J Pathol. 164: 665-77.
  9. Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
    PLoS One. 4: e7475.
  10. Ribeiro Dos Santos, P. et al. (2011) Rapid dissemination of SIV follows multisite entry after rectal inoculation.
    PLoS One. 6 (5): e19493.
  11. Kamihata, H. et al. (2001) Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines.
    Circulation. 104 (9): 1046-52.
  12. Riddell, M.R. et al. (2012) The characterization of fibrocyte-like cells: a novel fibroblastic cell of the placenta.
    Placenta. 33 (3): 143-50.
  13. Hung, C.H. et al. (2018) Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
    Sci Rep. 8 (1): 919.
  14. Hung, C.H. et al. (2018) Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer.
    BMC Cancer. 18 (1): 366.