Macrophage antibody | PM-2K
Mouse anti Human macrophages, clone PM-2K does not react with macrophage scavenger receptors AI and AII.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Dog Hamster Guinea Pig Pig Monkey Horse Cat Rat Bovine Goat Rabbit
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Kathon™
0.5% Bovine Serum Albumin
- Cultured human peritoneal macrophages.
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml after reconstitution
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
Applications of Macrophage antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1||1/200||1/800|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Histology Positive Control Tissue
- Western Blotting
- 5870-8008 recognizes an antigen of 150 kDa on macrophage cell membranes.
Secondary Antibodies Available
Product Specific References
Takeya, M. et al. (1991) A new monoclonal antibody, PM-2K, specifically recognizes tissue macrophages but not blood monocytes.
J Pathol. 163 (4): 315-21.
References for Macrophage antibody
Frangogiannis, N.G. et al. (2002) Evidence for an active inflammatory process in the hibernating human myocardium.
Am J Pathol. 160: 1425-33.
Kume, S. et al. (1995) Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta with a novel specific monoclonal antibody.
Am J Pathol. 147: 654-67.
Frangogiannis, N.G. et al. (2003) MCSF expression is induced in healing myocardial infarcts and may regulate monocyte and endothelial cell phenotype.
Am J Physiol Heart Circ Physiol. 285: H483-92.
Somasundaram, P. et al. (2005) Mast cell tryptase may modulate endothelial cell phenotype in healing myocardial infarcts.
J Pathol. 205: 102-11.
Zeng, L. et al. (1996) Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
J Histochem Cytochem. 44: 845-53.
Horiuchi, S. et al. (1996) Extra- and intracellular localization of advanced glycation end-products in human atherosclerotic lesions.
Nephrol Dial Transplant. 11 Suppl 5: 81-6.
Dobaczewski, M. et al. (2006) Extracellular matrix remodeling in canine and mouse myocardial infarcts.
Cell Tissue Res. 324: 475-88.
Dewald, O. et al. (2004) Of mice and dogs: species-specific differences in the inflammatory response following myocardial infarction
Am J Pathol. 164: 665-77.
Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
PLoS One. 4: e7475.
Ribeiro Dos Santos, P. et al. (2011) Rapid dissemination of SIV follows multisite entry after rectal inoculation.
PLoS One. 6 (5): e19493.
Kamihata, H. et al. (2001) Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines.
Circulation. 104 (9): 1046-52.
Riddell, M.R. et al. (2012) The characterization of fibrocyte-like cells: a novel fibroblastic cell of the placenta.
Placenta. 33 (3): 143-50.
Hung, C.H. et al. (2018) Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
Sci Rep. 8 (1): 919.
Hung, C.H. et al. (2018) Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer.
BMC Cancer. 18 (1): 366.
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