Macrophage antibody | PM-2K
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used for the demonstration of PM-2K expressing cells by immunocytochemistry.
Image caption:
Expression of markers on macrophages cultured in serum and M-CSF.
PBMC were cultured in SFM at 5×105 cells/ml in 8-well glass slides for 7 days in the presence of 10% FCS and 10 ng/ml M-CSF. Cells were then air-dried, fixed and stained with 5 μg/ml mouse IgG1 monoclonal antibodies against CD13, CD14, CD32, CD163, CD90, mouse IgG1, proyly-4-hydroxylase, 25F9, S100A8/A9, PM-2K, TE-7, and collagen-I. Cells were then counterstained with hematoxylin to identify nuclei. Inserts indicate staining at higher magnification. Bar is 50 μm.
From: Pilling D, Fan T, Huang D, Kaul B, Gomer RH (2009)
Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts.
PLoS ONE 4(10): e7475.
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used for the demonstration of tissue macrophages in lung sections by immunofluorescence.
Image caption:
Identification of fibrocytes in pathological samples.
“HOPE-fixed” human lung tissue sections were stained with antibodies against CD32, CD45RO, CD68, CD164, collagen-I, PM-2K, and TE-7. Sections were stained for A) CD45RO (green), CD68 (red) and DAPI (blue). An example of a CD45RO positive lymphocyte is indicated with a solid arrow. Dual labeled CD45RO and CD68 macrophages and fibrocytes are indicated by open arrows. B) CD45RO (green), PM-2K (red) and DAPI (blue). An example of a dual labeled CD45RO and PM-2K tissue macrophage is indicated with a solid arrow. C) CD45RO (green), collagen-I (red), and DAPI (blue). Dual labeled CD45RO and collagen-I positive cells are yellow. D) PM-2K (green), collagen-I (red), and DAPI (blue). A cluster of PM-2K positive alveolar macrophages is indicated with an open arrow. E) CD45RO (green), CD164 (red), and DAPI (blue). An area of CD164 positive fibroblasts is indicated with a solid arrow. A dual labeled CD45RO and CD164 positive fibrocyte is indicated by an open arrow. F) CD45RO (green), collagen-I (red), and TE-7 (blue). Dual positive TE-7/collagen-I fibroblasts are magenta. G) TE-7 (green), CD32 (red), and DAPI (blue). An example of CD32 positive fibrocytes is indicated with an open arrow. H) Nuclei (white), PM-2K (green), collagen (red), and CD32 (blue). A cluster of PM-2K positive alveolar macrophages are indicated with an asterisk. An area of CD32 and collagen positive/PM-2K negative fibrocytes is indicated with an open arrow. An area of collagen positive/CD32 negative fibroblasts is indicated with a solid arrow. Cells were counterstained with DAPI to identify nuclei. Bars are 100 μm.
From: Pilling D, Fan T, Huang D, Kaul B, Gomer RH (2009)
Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts.
PLoS ONE 4(10): e7475.
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used for identifying tissue macrpohages in macaque tissue sections by immunofluorescence.
Image caption:
DC-SIGN+ cells are macrophages in the colo-rectal mucosa during the first sixteen hours of infection. DC-SIGN+ cells are observed in lymphoid aggregates of R-H4.2 (sacrificed four hours pi; A) and lamina propria of R-H4.1 (sacrificed four hours pi; C) and R-H16.3 (sacrificed sixteen hours pi; B and D). DC-SIGN+ cells express only occasionally MHC-II molecules (A and B, arrows), but co-express PM-2K (C) and CD68 (D) marking them as macrophages. Frozen sections of colo-rectal mucosa were labeled for DC-SIGN (Alexa-488), CD3 (Alexa-350, A, B and D), MHC-II Mamula-DR (Alexa-546, A, B and C) or PM-2K (Alexa-350, C), CD68 (Alexa-546, D) and nuclei were stained with TO-PRO®-3 (A–D). The computer-generated merged images are shown. CR, crypt; LP, lamina propria; S, submucosal connective tissue; TA, T cell area of mucosal lymphoid aggregate; HEV, high endothelial venule.
From: Ribeiro dos Santos P, Rancez M, Prétet J-L, Michel-Salzat A, Messent V, Bogdanova A, et al. (2011)
Rapid Dissemination of SIV Follows Multisite Entry after Rectal Inoculation.
PLoS ONE 6(5): e19493.
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used to evaluate expression levels on circulating PBMC by flow cytometry
Image caption:
Analysis of circulating fibrocytes and PM-2K+ cells in peripheral blood mononuclear cells. (a) Representative dot plots of the respective dataset from normal and asthmatic patient. The intracellular collagen I+ (fibrocytes) and PM-2K+ cells are gated from viable CD3−CD19−CD45+ cells. (b) Their relationship was analyzed by linear regression based on the logarithm of percentages of fibrocytes or PM-2K+ cells in each individual. Normal, N = 92, Asthma, N = 48.
From: Hung CH, Wang CC, Suen JL, Sheu CC, Kuo CH, Liao WT, Yang YH, Wu CC, Leung SY, Lai RS, Lin CC, Wei YF, Lee CY, Huang MS, Huang SK.
Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
Sci Rep. 2018 Jan 17;8(1):919. doi: 10.1038/s41598-017-19105-z.
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used to evaluate PM-2K expression levels on circulating PBMC by flow cytometry.
Image caption:
Identification of circulating PM-2K+ cells and its subsets. (a) The sequential gating strategy was shown on live and small cells (left), single cells (middle) and non-T/non-B cells (right). (b–d) PM-2K+ cells were gated against fluorescence minus one (FMO) control to identify the CCR7+CD86+ (purple gate) and CCR7−CD86+ (orange gate) subsets in PM-2K+CD14+ (green gate) and PM-2K+CD14− (red gate) cells, using appropriate FMO controls. (e) Confocal imaging analysis of CD14+ cells from two normal subjects; PM-2K (green) and nucleus (blue).
From: Hung CH, Wang CC, Suen JL, Sheu CC, Kuo CH, Liao WT, Yang YH, Wu CC, Leung SY, Lai RS, Lin CC, Wei YF, Lee CY, Huang MS, Huang SK.
Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
Sci Rep. 2018 Jan 17;8(1):919. doi: 10.1038/s41598-017-19105-z.
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used to evaluate expression levels on circulating PBMC by flow cytometry
Image caption:
PM-2K+ cells in peripheral blood. Ficoll-isolated PBMCs from one normal individual were stained with PM-2K antibody, followed by FITC anti-mouse IgG. After washing, cells were then stained with Alexa700 anti-CD14. PM-2K expression was shown in the “monocyte” gate (green) (B) or “small cell” gate (red) (C). The boundary between PM-2K+ and PM-2K- cells was set based on the fluorescence minus one control specific for PM-2K
From: Hung CH, Wang CC, Suen JL, Sheu CC, Kuo CH, Liao WT, Yang YH, Wu CC, Leung SY, Lai RS, Lin CC, Wei YF, Lee CY, Huang MS, Huang SK.
Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
Sci Rep. 2018 Jan 17;8(1):919. doi: 10.1038/s41598-017-19105-z.
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used for the evaluation and selection of peripheral blood mononuclear cells by flow cytometry.
Image caption:
The gating strategy and histograms with the gating of all the antibodies by flow cytometry. Sample data from a patient with breast cancer are shown. Live cells were gated on a forward scatter (FSC)/side scatter (SSC) plot (A). These cells were then further gated to determine CD3−, CD19− (B), PM-2 K+ macrophages (C). Macrophages were further gated to determine CCR7+CD86+ M1-like macrophages, CCR7−CXCR1+ M2a-like macrophages, CCR7−CD86+ M2b-like macrophages, and CCR7−CCR2+ M2c-like macrophages in PM-2 K+CD14+ (D) and PM-2 K+CD14− (E) groups. (PDF
From: Hung CH, Chen FM, Lin YC, Tsai ML, Wang SL, Chen YC, Chen YT, Hou MF.
Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer.
BMC Cancer. 2018 Apr 3;18(1):366.
Mouse anti Human macrophages, clone PM-2K recognizes most tissue macrophages in thymus, spleen, lymph nodes and tonsil. It is positive on Kupffer cells (liver), alveolar macrophages and macrophages in the interstitial tissue of the kidney, pancreas and other organs.
Mouse anti Human macrophages, clone PM-2K does not react with macrophage scavenger receptors AI and AII.
Mouse anti Human macrophages, clone PM-2K does not react with macrophage scavenger receptors AI and AII.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Hamster Guinea Pig Pig Monkey Horse Cat Rat Bovine Goat Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Kathon™
0.5% Bovine Serum Albumin - Immunogen
- Cultured human peritoneal macrophages.
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml after reconstitution
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of Macrophage antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen 1 | 1/200 | 1/800 | |
| Immunohistology - Paraffin | |||
| Western Blotting |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil
- Western Blotting
- 5870-8008 recognizes an antigen of 150 kDa on macrophage cell membranes.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Source Reference
-
Takeya, M. et al. (1991) A new monoclonal antibody, PM-2K, specifically recognizes tissue macrophages but not blood monocytes.
J Pathol. 163 (4): 315-21.
References for Macrophage antibody
-
Frangogiannis, N.G. et al. (2002) Evidence for an active inflammatory process in the hibernating human myocardium.
Am J Pathol. 160: 1425-33. -
Kume, S. et al. (1995) Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta with a novel specific monoclonal antibody.
Am J Pathol. 147: 654-67. -
Frangogiannis, N.G. et al. (2003) MCSF expression is induced in healing myocardial infarcts and may regulate monocyte and endothelial cell phenotype.
Am J Physiol Heart Circ Physiol. 285: H483-92. -
Somasundaram, P. et al. (2005) Mast cell tryptase may modulate endothelial cell phenotype in healing myocardial infarcts.
J Pathol. 205: 102-11. -
Zeng, L. et al. (1996) Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
J Histochem Cytochem. 44: 845-53. -
Horiuchi, S. et al. (1996) Extra- and intracellular localization of advanced glycation end-products in human atherosclerotic lesions.
Nephrol Dial Transplant. 11 Suppl 5: 81-6. -
Dobaczewski, M. et al. (2006) Extracellular matrix remodeling in canine and mouse myocardial infarcts.
Cell Tissue Res. 324: 475-88. -
Dewald, O. et al. (2004) Of mice and dogs: species-specific differences in the inflammatory response following myocardial infarction
Am J Pathol. 164: 665-77. -
Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
PLoS One. 4: e7475. -
Ribeiro Dos Santos, P. et al. (2011) Rapid dissemination of SIV follows multisite entry after rectal inoculation.
PLoS One. 6 (5): e19493. -
Kamihata, H. et al. (2001) Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines.
Circulation. 104 (9): 1046-52. -
Riddell, M.R. et al. (2012) The characterization of fibrocyte-like cells: a novel fibroblastic cell of the placenta.
Placenta. 33 (3): 143-50. -
Hung, C.H. et al. (2018) Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
Sci Rep. 8 (1): 919. -
Hung, C.H. et al. (2018) Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer.
BMC Cancer. 18 (1): 366.
