Macrophage antibody | PM-2K







Mouse anti Human macrophages, clone PM-2K does not react with macrophage scavenger receptors AI and AII.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Hamster Guinea Pig Pig Monkey Horse Cat Rat Bovine Goat Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Kathon™
0.5% Bovine Serum Albumin - Immunogen
- Cultured human peritoneal macrophages.
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml after reconstitution
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Macrophage antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen 1 | 1/200 | 1/800 | |
Immunohistology - Paraffin | |||
Western Blotting |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Histology Positive Control Tissue
- Tonsil
- Western Blotting
- 5870-8008 recognizes an antigen of 150 kDa on macrophage cell membranes.
Secondary Antibodies Available
Product Specific References
Source Reference
-
Takeya, M. et al. (1991) A new monoclonal antibody, PM-2K, specifically recognizes tissue macrophages but not blood monocytes.
J Pathol. 163 (4): 315-21.
References for Macrophage antibody
-
Frangogiannis, N.G. et al. (2002) Evidence for an active inflammatory process in the hibernating human myocardium.
Am J Pathol. 160: 1425-33. -
Kume, S. et al. (1995) Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta with a novel specific monoclonal antibody.
Am J Pathol. 147: 654-67. -
Frangogiannis, N.G. et al. (2003) MCSF expression is induced in healing myocardial infarcts and may regulate monocyte and endothelial cell phenotype.
Am J Physiol Heart Circ Physiol. 285: H483-92. -
Somasundaram, P. et al. (2005) Mast cell tryptase may modulate endothelial cell phenotype in healing myocardial infarcts.
J Pathol. 205: 102-11. -
Zeng, L. et al. (1996) Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
J Histochem Cytochem. 44: 845-53. -
Horiuchi, S. et al. (1996) Extra- and intracellular localization of advanced glycation end-products in human atherosclerotic lesions.
Nephrol Dial Transplant. 11 Suppl 5: 81-6. -
Dobaczewski, M. et al. (2006) Extracellular matrix remodeling in canine and mouse myocardial infarcts.
Cell Tissue Res. 324: 475-88. -
Dewald, O. et al. (2004) Of mice and dogs: species-specific differences in the inflammatory response following myocardial infarction
Am J Pathol. 164: 665-77. -
Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
PLoS One. 4: e7475. -
Ribeiro Dos Santos, P. et al. (2011) Rapid dissemination of SIV follows multisite entry after rectal inoculation.
PLoS One. 6 (5): e19493. -
Kamihata, H. et al. (2001) Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines.
Circulation. 104 (9): 1046-52. -
Riddell, M.R. et al. (2012) The characterization of fibrocyte-like cells: a novel fibroblastic cell of the placenta.
Placenta. 33 (3): 143-50. -
Hung, C.H. et al. (2018) Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.
Sci Rep. 8 (1): 919. -
Hung, C.H. et al. (2018) Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer.
BMC Cancer. 18 (1): 366.
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