LYVE-1 antibody | 4G1
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WB ResetMouse anti Human LYVE-1
- Product Type
- Monoclonal Antibody
- Clone
- 4G1
- Isotype
- IgG2b
- Specificity
- LYVE-1
Antibody Quality and 
Unrivaled 
| Mouse anti human LYVE-1, clone 4G1 recogniszs human LYVE-1, otherwise known as hyaluronic acid receptor, a type I integral membrane glycoprotein and member of the Link protein superfamily, expressed on the cell surface of both normal and neoplastic lymphatic endothelial cells. LYVE-1 acts a major receptor for the glycosaminoglycan hyaluronan (HA) in both its soluble and immobilized forms. The binding of LYVE-1 to HA on lymph vessel walls and not to the endothelium of blood vessels, makes LYVE-1 a powerful marker for the identification of lymphatic endothelial cells, and for distinguishing between lymphatic vessels and blood vasculature (Koukourakis et al. 2005). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
10% Glycerol - Immunogen
- Recombinant human LYVE-1 (amino acids 25-235) purified from Escherichia coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA4629GA detects a band of approximately 38 kDa in human A549 (lung carcinoma) cell lysates.
Further Reading
-
Banerji, S. et al. (1999) LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.
J Cell Biol. 144 (4): 789-801.
- Synonyms
- Hyaluronic Acid Receptor
- RRID
- AB_2138660
- UniProt
- Q9Y5Y7
- Entrez Gene
- LYVE1
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005624 membrane fraction
- GO:0006810 transport
- GO:0006928 cellular component movement
- GO:0007160 cell-matrix adhesion
- GO:0009653 anatomical structure morphogenesis
- GO:0009611 response to wounding
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