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Lysine-Specific Demethylase 3A antibody

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Western Blot Validated
Anti Lysine-Specific Demethylase 3A Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with lysine-specific demethylase 3A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 40 second exposure. Arrow points to lysine-specific demethylase 3A (molecular weight 150 kDa)

Mouse anti Lysine-Specific Demethylase 3A

Lysine-specific demethylase 3A antibody recognizes lysine-specific demethylase 3A, also known as KDM3A, JmjC domain-containing histone demethylation protein 2A, jumonji C domain-containing histone demethylase 2A, or jumonji domain containing 1.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00290 WB 100 µl
Mouse anti Human lysine-specific demethylase 3A antibody recognizes lysine-specific demethylase 3A, also known as jmjC domain-containing histone demethylation protein 2A, jumonji C domain-containing histone demethylase 2A, jumonji domain containing 1, jumonji domain containing 1A and testis-specific protein A.

Encoded by the KDM3A gene, lysine-specific demethylase 3A is a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. (provided by RefSeq, Apr 2009).

Mouse anti Human lysine-specific demethylase 3A antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti lysine-specific demethylase 3A detects a band of approximately 150 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human lysine-specific demethylase 3A expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9Y4C1 Related reagents
Entrez Gene
KDM3A Related reagents
GO Terms
GO:0005506 iron ion binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0055114 oxidation-reduction process
GO:0006350 transcription
GO:0007283 spermatogenesis
GO:0009755 hormone-mediated signaling pathway
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0030154 cell differentiation
GO:0030521 androgen receptor signaling pathway
GO:0033169 histone H3-K9 demethylation
GO:0045941 positive regulation of transcription
GO:0046293 formaldehyde biosynthetic process
GO:0050681 androgen receptor binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Lysine-Specific Demethylase 3A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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