Lysine-Specific Demethylase 3A antibody

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Mouse anti Lysine-Specific Demethylase 3A

Lysine-specific demethylase 3A antibody recognizes lysine-specific demethylase 3A, also known as KDM3A, JmjC domain-containing histone demethylation protein 2A, jumonji C domain-containing histone demethylase 2A, or jumonji domain containing 1.

Lysine-Specific Demethylase 3A Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00290	WB	100 µl
VMA00290KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human lysine-specific demethylase 3A antibody recognizes lysine-specific demethylase 3A, also known as jmjC domain-containing histone demethylation protein 2A, jumonji C domain-containing histone demethylase 2A, jumonji domain containing 1, jumonji domain containing 1A and testis-specific protein A.

Encoded by the KDM3A gene, lysine-specific demethylase 3A is a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. (provided by RefSeq, Apr 2009).

Mouse anti Human lysine-specific demethylase 3A antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti lysine-specific demethylase 3A detects a band of approximately 150 kDa in Jurkat cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation: 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Purified recombinant fragment of human lysine-specific demethylase 3A expressed in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q9Y4C1 Related reagents
Entrez Gene: KDM3A Related reagents
GO Terms: GO:0005506 iron ion binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0055114 oxidation-reduction process; GO:0006350 transcription; GO:0007283 spermatogenesis; GO:0009755 hormone-mediated signaling pathway; GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; GO:0030154 cell differentiation; GO:0030521 androgen receptor signaling pathway; GO:0033169 histone H3-K9 demethylation; GO:0045941 positive regulation of transcription; GO:0046293 formaldehyde biosynthetic process; GO:0050681 androgen receptor binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Lysine-Specific Demethylase 3A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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