Lyn antibody | LYN01

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Mouse anti Lyn

Product Type
Monoclonal Antibody
Clone
LYN01
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2424 IF IP WB 0.1 mg
Mouse anti Human lyn antibody, clone LYN01 recognizes Tyrosine-protein kinase Lyn, also known as p56lyn, p53lyn, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog or Lck/Yes-related novel protein tyrosine kinase a 512 amino acid, ~56 kDa member of the src family of non-receptor protein tyrosine kinases (PTKs), predominantly expressed in tissues of haematopoietic origin.

The interaction between lyn and syk, is crucial for regulating the response of both mast cells and basophils, following Fc epsilon R1 receptor aggregation and activation. Once activated, the rapid tyrosine phosphorylation of ITAMs by lyn, serves as a binding site for the recruitment and activation of syk, triggering the phosphorylation of further adaptor and signaling molecules further down a complex signaling cascade. This chain of events involving receptor aggregation, activation and tyrosine phosphorylation of ITAMs, is also vital for the survival and differentiation of B cells through the interaction of lyn and syk with the B cell receptor (BCR).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant fragment of human Lyn (amino acids 8-238)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07948 Related reagents
Entrez Gene
LYN Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
GO:0006974 response to DNA damage stimulus
GO:0008284 positive regulation of cell proliferation
GO:0009725 response to hormone stimulus
GO:0030168 platelet activation
GO:0030218 erythrocyte differentiation
GO:0031295 T cell costimulation
GO:0042531 positive regulation of tyrosine phosphorylation of STAT protein
GO:0044419 interspecies interaction between organisms
GO:0045121 membrane raft
GO:0048471 perinuclear region of cytoplasm
GO:0050900 leukocyte migration
GO:0051272 positive regulation of cellular component movement
GO:0070304 positive regulation of stress-activated protein kinase signaling cascade
Regulatory
For research purposes only

Applications of Lyn antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunoprecipitation
Western Blotting 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2424 detects a band of approximately 53-56kDa in human peripheral blood monocytes.

Product Specific References

References for Lyn antibody

  1. Amoui, M. et al. (1997) Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors.
    Eur J Immunol. 27 (1): 321-8.
  2. Dráberová, L. et al. (1996) Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains.
    Immunology. 87 (1): 141-8.

Further Reading

  1. Kovárová, M. et al. (2001) Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation.
    Mol Cell Biol. 21 (24): 8318-28.