Lyn antibody | AbD02974

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Human anti Human Lyn

Product Type
Monoclonal Antibody
Clone
AbD02974
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA026 E WB 0.1 mg
Human anti Human Lyn antibody, clone AbD02974 recognizes the human tyrosine-protein kinase Lyn, a 512 amino acid ~56 kDa member of the Src family of kinases containing single protein kinase, SH2 and SH3 domains. Lyn is also known as Lck/Yes-related novel protein tyrosine kinase, p53Lyn or p56Lyn.

Lyn is predominantly expressed in hematopoietic cells, and is involved in signaling from cell surface receptors such as CD40 and sIg. Lyn plays multiple roles in processes such a proliferation, B-cell regulation and autoimmunity (Ingley 2012). Lyn expression has also been reported on human malignant lymphomas (Choi et al. 1993).

Product Details

Target Species
Human
Product Form
A lyophilized, bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
Reconstitution
Reconstitute with 0.1 ml distilled water
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human SRC
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml following reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of reconstitution

More Information

UniProt
P07948 Related reagents
Entrez Gene
LYN Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
GO:0006974 response to DNA damage stimulus
GO:0008284 positive regulation of cell proliferation
GO:0009725 response to hormone stimulus
GO:0030168 platelet activation
GO:0030218 erythrocyte differentiation
GO:0031295 T cell costimulation
GO:0042531 positive regulation of tyrosine phosphorylation of STAT protein
GO:0044419 interspecies interaction between organisms
GO:0045121 membrane raft
GO:0048471 perinuclear region of cytoplasm
GO:0050900 leukocyte migration
GO:0051272 positive regulation of cellular component movement
GO:0070304 positive regulation of stress-activated protein kinase signaling cascade
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Lyn antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB