LMO2 antibody | 1A9-1

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Mouse anti Human LMO2

Product Type
Monoclonal Antibody
Clone
1A9-1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2744GA IF P* WB 0.1 mg
Mouse anti Human LMO2 antibody, clone 1A9-1 recognizes the human Rhombotin-2 also known as LMO2 or T-cell translocation protein 2. LMO2 is a 158 amino acid ~24kD transcription factor containing 2 LIM domains that interacts with DNA binding proteins such as TAL1/SCL and GATA1. In early development, LMO2 is expressed in the central nervous system (Herberth et al. 2005) and a wide range of tissue, and in adults in myeloid and erythroid precursors, in normal germinal centre B-cells, endothelial cells and parts of the brain (Natkunam et al. 2006). LMO2 plays a vital role in early stages of haematopoiesis and in regulating yolk-sac erythropoiesis in mice (Kathleen et al. 2005). Studies suggest that it may also play a role in angiogenesis (Yamada et al. 2002).

A major cause of T-cell acute lymphoblastic leukemia (T-ALL) is the aberrant activation of the TAL1/SCL, LMO1/2, and NOTCH1 oncogenes (Ferrando et al. 2004). Activation of the Notch signalling pathway by mutations in either the gamma-secretase or PEST domains are found in more than 50% of all T-ALL, including LMO2-induced T-ALL, and studies using mouse models, have show that activation of Notch co-operates with Scl/Lmo1 in inducing T-ALL (Weng et al. 2004). Aberrant expression of LMO2 has also been linked to certain types of B-cell lymphoma (Natkunam et al. 2008) and prostate cancer, with overexpression possibly linked to cancer progression (Ma et al. 2007).

Mouse anti human LMO2, clone 1A9-1 has been used for the detection of LMO2 by immunohistochemistry on formalin fixed, paraffin embedded tissues and by Western blotting (Natkunam et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified GST-LMO2 fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised mice were fused with cells of the K6H6B5 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P25791 Related reagents
Entrez Gene
LMO2 Related reagents
GO Terms
GO:0005634 nucleus
GO:0008270 zinc ion binding
Regulatory
For research purposes only

Applications of LMO2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil
Western Blotting
MCA2744GA detects a band of approximately 24kDa in Raji cell lysates.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for LMO2 antibody

  1. Natkunam, Y. et al. (2007) The oncoprotein LMO2 is expressed in normal germinal-center B cells and in human B-cell lymphomas.
    Blood. 109 (4): 1636-42.
  2. Sewell, H. et al. (2014) Conformational flexibility of the oncogenic protein LMO2 primes the formation of the multi-protein transcription complex.
    Sci Rep. 4:3643.
  3. Younes, S.F. et al. (2011) The efficacy of HGAL and LMO2 in the separation of lymphomas derived from small B cells in nodal and extranodal sites, including the bone marrow.
    Am J Clin Pathol. 135: 697-708.