LIMK1 antibody | OTI6B4
There are approximately 40 known eukaryotic LIM proteins, named after the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.
LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuo-spatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Feb 2011).
Mouse anti Human LIMK1 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti LIMK1 detects a band of approximately 70 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Full length recombinant human LIMK1 (NP_002305) produced in HEK293T cells
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- Entrez Gene
- GO Terms
- GO:0030036 actin cytoskeleton organization
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006468 protein phosphorylation
- GO:0007266 Rho protein signal transduction
- GO:0007411 axon guidance
- GO:0008270 zinc ion binding
- GO:0031072 heat shock protein binding
- GO:0030426 growth cone
- GO:0032233 positive regulation of actin filament bundle assembly
- GO:0045773 positive regulation of axon extension
- GO:0051444 negative regulation of ubiquitin-protein ligase activity
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of LIMK1 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|