LEF1 antibody | 1866CT359.68.92.75

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Mouse anti LEF1

LEF1 antibody recognizes the lymphoid enhancer-binding factor 1, also known as T cell-specific transcription factor 1-alpha, or TCF1-alpha.

LEF1 Antibody with Control Lysate

LEF1 antibody recognizes the lymphoid enhancer-binding factor 1, also known as T cell-specific transcription factor 1-alpha, or TCF1-alpha.

Product Type
PrecisionAb™ Monoclonal
Clone
1866CT359.68.92.75
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00706 WB 100 µl
VMA00706KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human LEF1 antibody recognizes the lymphoid enhancer-binding factor 1, also known as T cell-specific transcription factor 1-alpha and TCF1-alpha.

LEF1 encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by LEF1 can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in LEF1 have been found in somatic sebaceous tumors. LEF1 has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants (provided by RefSeq, Oct 2009).

Mouse anti Human LEF1 antibody detects bands of 39, 42 and 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti LEF1 detects bands of approximately 39, 42 & 58 kDa in Jurkat cell lysates
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Recombinant protein of human LEF1 (amino acids 115-283)
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9UJU2 Related reagents
Entrez Gene
LEF1 Related reagents
GO Terms
GO:0001649 osteoblast differentiation
GO:0001837 epithelial to mesenchymal transition
GO:0042393 histone binding
GO:0005737 cytoplasm
GO:0005667 transcription factor complex
GO:0008013 beta-catenin binding
GO:0008284 positive regulation of cell proliferation
GO:0008301 DNA bending activity
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010718 positive regulation of epithelial to mesenchymal transition
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0021987 cerebral cortex development
GO:0022408 negative regulation of cell-cell adhesion
GO:0022409 positive regulation of cell-cell adhesion
GO:0030223 neutrophil differentiation
GO:0030284 estrogen receptor activity
GO:0030307 positive regulation of cell growth
GO:0030331 estrogen receptor binding
GO:0030335 positive regulation of cell migration
GO:0030854 positive regulation of granulocyte differentiation
GO:0032582 negative regulation of gene-specific transcription
GO:0032696 negative regulation of interleukin-13 production
GO:0032713 negative regulation of interleukin-4 production
GO:0032714 negative regulation of interleukin-5 production
GO:0032993 protein-DNA complex
GO:0035326 enhancer binding
GO:0043027 caspase inhibitor activity
GO:0043154 negative regulation of caspase activity
GO:0043392 negative regulation of DNA binding
GO:0043923 positive regulation by host of viral transcription
GO:0043966 histone H3 acetylation
GO:0043967 histone H4 acetylation
GO:0045063 T-helper 1 cell differentiation
GO:0045295 gamma-catenin binding
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0060021 palate development
GO:0060070 canonical Wnt receptor signaling pathway
GO:0060326 cell chemotaxis
GO:0070016 armadillo repeat domain binding
GO:0070742 C2H2 zinc finger domain binding
GO:0071353 cellular response to interleukin-4
GO:0071864 positive regulation of cell proliferation in bone marrow
GO:0071866 negative regulation of apoptosis in bone marrow
GO:0071899 negative regulation of estrogen receptor binding
GO:0090068 positive regulation of cell cycle process
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of LEF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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