Lck antibody

100% Secure


Mouse anti Lck

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00382
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human LCK antibody recognizes the tyrosine-protein kinase Lck, also known as T-lymphocyte specific protein tyrosine kinase p56lck, leukocyte C-terminal Src kinase and T cell-specific protein-tyrosine kinase.

LCK is a member of the Src family of protein tyrosine kinases (PTKs). The LCK gene encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described (provided by RefSeq, Jul 2008).

Mouse anti Human LCK antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti LCK detects a band of approximately 56 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human LCK expressed in E.coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P06239 Related reagents
Entrez Gene
LCK Related reagents
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Lck antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.