Laminin antibody | 5H2

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Mouse anti Human Laminin Alpha 2

Product Type
Monoclonal Antibody
Clone
5H2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
6067-0906 C* E IF IP P WB 50 µl
Mouse anti Human laminin alpha 2 antibody, clone 5H2 recognizes the ~80 kDa fragment of laminin subunit alpha-2, also known as the laminin M chain, laminin-12 subunit alpha, laminin-2 subunit alpha, laminin-4 subunit alpha or merosin heavy chain. Laminins are trimeric basement glycoproteins consisting of three non-identical multi-domain chains (alpha, beta and gamma) each encoded by a distinct gene. The alpha-2 chain is a subunit of Laminin-2 and Laminin-4, widely expressed in the basement membrane of skeletal muscle and peripheral nerves. Laminins are thought to mediate cell attachment, migration, proliferation and differentiation with other extracellular matrix components.

Mutation of the LAMA2 gene can lead to the development of Merosin-deficient congenital muscular dystrophy 1A (MDC1A), a condition characterized by hypotonia, proximal weakness, hyporeflexia and difficulty walking (Tazek et al. 2003).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascitic Fluid - raw
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified Human Merosin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P24043 Related reagents
Entrez Gene
LAMA2 Related reagents
GO Terms
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0005198 structural molecule activity
GO:0005606 laminin-1 complex
GO:0007517 muscle organ development
GO:0030155 regulation of cell adhesion
GO:0030334 regulation of cell migration
GO:0045995 regulation of embryonic development
Regulatory
For research purposes only

Applications of Laminin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/25000 1/100000
Immunofluorescence
Immunohistology - Frozen 1 1/2000 1/10000
Immunohistology - Paraffin 1/50
Immunoprecipitation
Western Blotting
  1. 1Acetone is recommended. 8 ┬Ám fixed cryostat muscle sections have been used, with an HRP conjugated secondary antibody for detection.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Laminin antibody

  1. Sewry, C.A. et al. (1995) Expression of laminin subunits in human fetal skeletal muscle.
    Histochem J. 27 (7): 497-504.
  2. Engvall, E. et al. (1990) Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
    Cell Regul. 1 (10): 731-40.
  3. Tan, E. et al. (1997) Late onset muscular dystrophy with cerebral white matter changes due to partial merosin deficiency.
    Neuromuscul Disord. 7 (2): 85-9.
  4. Awamura, Y. et al. (2008) Long-term follow-up of laminin alpha2 (merosin)-deficient muscular dystrophy in a cat.
    J Feline Med Surg. 10 (3): 274-9.
  5. Eriksson, A. et al. (2005) Skeletal muscle morphology in power-lifters with and without anabolic steroids.
    Histochem Cell Biol. 124 (2): 167-75.
  6. Guo, L.T. et al. (2003) Laminin alpha2 deficiency and muscular dystrophy; genotype-phenotype correlation in mutant mice.
    Neuromuscul Disord. 13 (3): 207-15.
  7. Kjellgren, D. et al. (2004) Laminin isoforms in human extraocular muscles.
    Invest Ophthalmol Vis Sci. 45 (12): 4233-9.
  8. Li, J. et al. (2006) Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions.
    J Invest Dermatol. 126 (2): 432-40.
  9. Marinkovich, M.P. et al. (1992) The dermal-epidermal junction of human skin contains a novel laminin variant.
    J Cell Biol. 119 (3): 695-703.
  10. Nakano, J. et al. (2005) Laminin-induced autoimmune myositis in rats.
    J Neuropathol Exp Neurol. 64 (9): 790-6.
  11. O'Brien DP et al. (2001) Laminin alpha 2 (merosin)-deficient muscular dystrophy and demyelinating neuropathy in two cats.
    J Neurol Sci. 189 (1-2): 37-43.
  12. Tian, M. et al. (1997) Laminin-alpha2 chain-like antigens in CNS dendritic spines.
    Brain Res. 764 (1-2): 28-38.
  13. Tran, T. et al. (2006) Endogenous laminin is required for human airway smooth muscle cell maturation.
    Respir Res. 7: 117.
  14. Vainionpää, N. et al. (2007) Basement membrane protein distribution in LYVE-1-immunoreactive lymphatic vessels of normal tissues and ovarian carcinomas.
    Cell Tissue Res. 328 (2): 317-28.
  15. Vuoristo, S. et al. (2009) Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support.
    J Cell Mol Med. 13 (8B): 2622-33.
  16. Alhamidi, M. et al. (2017) Limb Girdle Muscular Dystrophy type 2I: The clinical variability seen in patients homozygous for the common FKRP (c.826C>A) mutation does not correlate with histopathological alterations, levels of glycosylated α-dystroglycan or laminin α2 in vastus lateralis.
    Neuromuscular Disorders. Mar 04 [Epub ahead of print]