Laminin antibody | 5H2
Mutation of the LAMA2 gene can lead to the development of Merosin-deficient congenital muscular dystrophy 1A (MDC1A), a condition characterized by hypotonia, proximal weakness, hyporeflexia and difficulty walking (Tazek et al. 2003).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rabbit Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ascitic Fluid - raw
- Preservative Stabilisers
0.09% Sodium Azide
- Purified Human Merosin.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005102 receptor binding
- GO:0007155 cell adhesion
- GO:0005198 structural molecule activity
- GO:0005606 laminin-1 complex
- GO:0007517 muscle organ development
- GO:0030155 regulation of cell adhesion
- GO:0030334 regulation of cell migration
- GO:0045995 regulation of embryonic development
- For research purposes only
Applications of Laminin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1||1/2000||1/10000|
|Immunohistology - Paraffin||1/50|
- 1Acetone is recommended. 8 µm fixed cryostat muscle sections have been used, with an HRP conjugated secondary antibody for detection.
Product Specific References
References for Laminin antibody
Sewry, C.A. et al. (1995) Expression of laminin subunits in human fetal skeletal muscle.
Histochem J. 27 (7): 497-504.
Engvall, E. et al. (1990) Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
Cell Regul. 1 (10): 731-40.
Tan, E. et al. (1997) Late onset muscular dystrophy with cerebral white matter changes due to partial merosin deficiency.
Neuromuscul Disord. 7 (2): 85-9.
Awamura, Y. et al. (2008) Long-term follow-up of laminin alpha2 (merosin)-deficient muscular dystrophy in a cat.
J Feline Med Surg. 10 (3): 274-9.
Eriksson, A. et al. (2005) Skeletal muscle morphology in power-lifters with and without anabolic steroids.
Histochem Cell Biol. 124 (2): 167-75.
Guo, L.T. et al. (2003) Laminin alpha2 deficiency and muscular dystrophy; genotype-phenotype correlation in mutant mice.
Neuromuscul Disord. 13 (3): 207-15.
Kjellgren, D. et al. (2004) Laminin isoforms in human extraocular muscles.
Invest Ophthalmol Vis Sci. 45 (12): 4233-9.
Li, J. et al. (2006) Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions.
J Invest Dermatol. 126 (2): 432-40.
Marinkovich, M.P. et al. (1992) The dermal-epidermal junction of human skin contains a novel laminin variant.
J Cell Biol. 119 (3): 695-703.
Nakano, J. et al. (2005) Laminin-induced autoimmune myositis in rats.
J Neuropathol Exp Neurol. 64 (9): 790-6.
O'Brien DP et al. (2001) Laminin alpha 2 (merosin)-deficient muscular dystrophy and demyelinating neuropathy in two cats.
J Neurol Sci. 189 (1-2): 37-43.
Tian, M. et al. (1997) Laminin-alpha2 chain-like antigens in CNS dendritic spines.
Brain Res. 764 (1-2): 28-38.
Tran, T. et al. (2006) Endogenous laminin is required for human airway smooth muscle cell maturation.
Respir Res. 7: 117.
Vainionpää, N. et al. (2007) Basement membrane protein distribution in LYVE-1-immunoreactive lymphatic vessels of normal tissues and ovarian carcinomas.
Cell Tissue Res. 328 (2): 317-28.
Vuoristo, S. et al. (2009) Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support.
J Cell Mol Med. 13 (8B): 2622-33.
Alhamidi, M. et al. (2017) Limb Girdle Muscular Dystrophy type 2I: The clinical variability seen in patients homozygous for the common FKRP (c.826C>A) mutation does not correlate with histopathological alterations, levels of glycosylated α-dystroglycan or laminin α2 in vastus lateralis.
Neuromuscular Disorders. Mar 04 [Epub ahead of print]
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