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Lamin A/C antibody | OTI3F6

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Anti Lamin A/C Antibody, clone OTI3F6 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Lamin A/C Antibody, clone OTI3F6 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Lamin A/C Antibody, clone OTI3F6 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Lamin A/C Antibody, clone OTI3F6 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with lamin A/C antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 73 second exposure. Arrow points to lamin A/C (molecular weight 63, 74 kDa)
Anti Lamin A/C Antibody, clone OTI3F6 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of Lamin A/C knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti Lamin A/C antibody (VMA00200) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 67 second and (B) 30 second exposure.
Anti Lamin A/C Antibody, clone OTI3F6 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of Lamin A/C from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti Lamin A/C

Product Type
PrecisionAb Monoclonal
Clone
OTI3F6
Isotype
IgG2b
Format
Purified
Specificity
Lamin A/C

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Product Code Applications Pack Size List Price Your Price Qty
VMA00200
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti Human lamin A/C antibody recognizes lamin A/C, also known as 70 kDa lamin, lamin, lamin A/C-like 1, prelamin-A/C and renal carcinoma antigen NY-REN-32.

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in the LMNA gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome (provided by RefSeq, Apr 2012).

Mouse anti Human lamin A/C antibody detects a bands of 63 and 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti lamin A/C detects a bands of approximately 63 and 74 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human lamin A/C (NP_733821) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P02545
Entrez Gene
LMNA
GO Terms
GO:0005515 protein binding
GO:0005198 structural molecule activity
GO:0005635 nuclear envelope
GO:0005638 lamin filament
GO:0005737 cytoplasm
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007517 muscle organ development
GO:0048471 perinuclear region of cytoplasm
GO:0090343 positive regulation of cell aging

VMA00200

240615

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