Lamin A/C antibody | JOL2

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Mouse anti Lamin A/C

Product Type
Monoclonal Antibody
Clone
JOL2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1429GA C F IF IP P* WB 0.1 mg
Mouse anti Human lamin A/C, clone JOL2, recognizes both recombinant and native forms of human lamin A and C, nuclear intermediate filament proteins encoded by the same LMNA gene via alternative splicing. In addition to providing structural support to the nuclear envelope, these proteins contribute to chromatin organisation and the regulation of gene expression.

Mutations in the LMNA gene are responsible for a broad spectrum of disorders, termed laminopathies, including Emery-Dreifuss muscular dystrophy and dilated cardiomyopathy.

The JOL2 clone has been shown to bind to an epitope between amino acids 464-572.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Xenopus
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human lamin A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2-0/Ag4 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02545 Related reagents
Entrez Gene
LMNA Related reagents
GO Terms
GO:0005515 protein binding
GO:0005198 structural molecule activity
GO:0005635 nuclear envelope
GO:0005638 lamin filament
GO:0005737 cytoplasm
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007517 muscle organ development
GO:0048471 perinuclear region of cytoplasm
GO:0090343 positive regulation of cell aging
Regulatory
For research purposes only

Applications of Lamin A/C antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 0.4 ug/ml
Immunoprecipitation
Western Blotting
  1. 1Mouse anti lamin A/C requires antigen retrieval using heat treatment prior to staining of paraffin sections. Citrate buffer pH 6.2 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human colon

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

References for Lamin A/C antibody

  1. Tilgner, K. et al. (2009) Dynamic complexes of A-type lamins and emerin influence adipogenic capacity of the cell via nucleocytoplasmic distribution of beta-catenin.
    J Cell Sci. 122: 401-13.
  2. Ivorra, C. et al. (2006) A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C.
    Genes Dev. 20: 307-20.
  3. Navarro, C.L. et al. (2004) Lamin A and ZMPSTE24 (FACE-1) defects cause nuclear disorganization and identify restrictive dermopathy as a lethal neonatal laminopathy.
    Hum Mol Genet. 13: 2493-503.
  4. Cummings, M. et al. (2006) XPA versus ERCC1 as chemosensitising agents to cisplatin and mitomycin C in prostate cancer cells: role of ERCC1 in homologous recombination repair.
    Biochem Pharmacol. 72: 166-75.
  5. Markiewicz, E. et al. (2006) The inner nuclear membrane protein emerin regulates beta-catenin activity by restricting its accumulation in the nucleus.
    EMBO J. 25: 3275-85.
  6. Zhang, Q. et al. (2007) Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrity.
    Hum Mol Genet. 16: 2816-33.
  7. Jean, D. et al. (2008) Cathepsin L expression is up-regulated by hypoxia in human melanoma cells: role of its 5'-untranslated region.
    Biochem J. 413: 125-34.
  8. Ndong, Jde. L. et al. (2009) Down-regulation of the expression of RB18A/MED1, a cofactor of transcription, triggers strong tumorigenic phenotype of human melanoma cells.
    Int J Cancer. 124: 2597-606.