Ku80 antibody | MEM-54

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Mouse anti Human Ku80

Product Type
Monoclonal Antibody
Clone
MEM-54
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2708 IP WB 0.1 mg
Mouse anti Human Ku80 antibody, clone MEM-54 recognizes the 80 kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination.

The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70 kDa (Ku70) and 80 kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.

Mouse anti Human Ku80 antibody, clone MEM-54 is suitable for use in western blotting (Jayaram et al. 2008).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Human T lymphocytes.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P13010 Related reagents
Entrez Gene
XRCC5 Related reagents
GO Terms
GO:0000723 telomere maintenance
GO:0000783 nuclear telomere cap complex
GO:0003690 double-stranded DNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0006350 transcription
GO:0042162 telomeric DNA binding
GO:0008022 protein C-terminus binding
GO:0010843 promoter binding
GO:0016481 negative regulation of transcription
GO:0019047 provirus integration
GO:0019059 initiation of viral infection
GO:0043564 Ku70:Ku80 complex
GO:0070419 nonhomologous end joining complex
Regulatory
For research purposes only

Applications of Ku80 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for Ku80 antibody

  1. Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.
    Nucleic Acids Res. 36: 5773-86.
  2. Krais, A.M. et al. (2011) CHRNA5 as negative regulator of nicotine signaling in normal and cancer bronchial cells: effects on motility, migration and p63 expression.
    Carcinogenesis. 32 (9): 1388-95.

Further Reading

  1. Koike, M. (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins.
    J Radiat Res. 43 (3): 223-36.