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Ku70 antibody

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Western Blot Validated
Anti Ku70 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Ku70 antibody (VMA00013) followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/5000, STAR126P) and visualized on the ChemiDoc MP with 20 second exposure. Arrow points to Ku70 (molecular weight 70 kDa)

Human anti Ku70

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00013
Datasheet
WB
MSDS
100 µl loader

Human anti Human Ku70 antibody recognizes Human Ku70, also known as X-ray repair cross-complementing protein 6 or XRCC6. Ku70 is a 609 amino acid, nuclear protein involved in DNA damage repair. Ku70 contains a single Ku domain and a single SAP domain.

Ku70 forms a heterodimer comprising of p70/p86 which acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA. Research indicates that the Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together (Walker et al. 2001).

Human anti Human Ku70 antibody recognizes human Ku70 as a single band of ~70 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti Ku70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human Ku70 - N1 fusion protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P12956 Related reagents
Entrez Gene
XRCC6 Related reagents
GO Terms
GO:0000723 telomere maintenance
GO:0000783 nuclear telomere cap complex
GO:0003690 double-stranded DNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0005624 membrane fraction
GO:0005667 transcription factor complex
GO:0005958 DNA-dependent protein kinase-DNA ligase 4 complex
GO:0006266 DNA ligation
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0006350 transcription
GO:0008022 protein C-terminus binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016481 negative regulation of transcription
GO:0016563 transcription activator activity
GO:0019047 provirus integration
GO:0019059 initiation of viral infection
GO:0043564 Ku70:Ku80 complex
GO:0051575 5'-deoxyribose-5-phosphate lyase activity
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Ku70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

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