Ku70 antibody

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Human anti Ku70

Ku70 antibody recognizes X-ray repair cross-complementing protein 6, also known as CTC75, CTCBF, DNA repair protein XRCC6, or thyroid-lupus autoantigen . The antibody has been extensively validated for western blotting using whole cell lysates.

Ku70 Antibody with Control Lysate

Ku70 antibody recognizes X-ray repair cross-complementing protein 6, also known as CTC75, CTCBF, DNA repair protein XRCC6, or thyroid-lupus autoantigen . The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00013 WB 100 µl
VMA00013KT WB 2 Westerns
With Lysate 400µg
Human anti Human Ku70 antibody recognizes Human Ku70, also known as X-ray repair cross-complementing protein 6 or XRCC6. Ku70 is a 609 amino acid, nuclear protein involved in DNA damage repair. Ku70 contains a single Ku domain and a single SAP domain.

Ku70 forms a heterodimer comprising of p70/p86 which acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA. Research indicates that the Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together (Walker et al. 2001).

Human anti Human Ku70 antibody recognizes human Ku70 as a single band of ~70 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti Ku70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Preparation
20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human Ku70 - N1 fusion protein
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P12956 Related reagents
Entrez Gene
XRCC6 Related reagents
GO Terms
GO:0000723 telomere maintenance
GO:0000783 nuclear telomere cap complex
GO:0003690 double-stranded DNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0005624 membrane fraction
GO:0005667 transcription factor complex
GO:0005958 DNA-dependent protein kinase-DNA ligase 4 complex
GO:0006266 DNA ligation
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0006350 transcription
GO:0008022 protein C-terminus binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016481 negative regulation of transcription
GO:0016563 transcription activator activity
GO:0019047 provirus integration
GO:0019059 initiation of viral infection
GO:0043564 Ku70:Ku80 complex
GO:0051575 5'-deoxyribose-5-phosphate lyase activity
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Ku70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB

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