Ku70 antibody
Human anti Human Ku70 antibody recognizes Human Ku70, also known as X-ray repair cross-complementing protein 6 or XRCC6. Ku70 is a 609 amino acid, nuclear protein involved in DNA damage repair. Ku70 contains a single Ku domain and a single SAP domain.
Ku70 forms a heterodimer comprising of p70/p86 which acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA. Research indicates that the Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together (Walker et al. 2001).
Human anti Human Ku70 antibody recognizes human Ku70 as a single band of ~70 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Ku70 forms a heterodimer comprising of p70/p86 which acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA. Research indicates that the Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together (Walker et al. 2001).
Human anti Human Ku70 antibody recognizes human Ku70 as a single band of ~70 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti Ku70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Preparation
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human Ku70 - N1 fusion protein
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P12956
- Entrez Gene
- XRCC6
- GO Terms
- GO:0000723 telomere maintenance
- GO:0000783 nuclear telomere cap complex
- GO:0003690 double-stranded DNA binding
- GO:0004003 ATP-dependent DNA helicase activity
- GO:0005524 ATP binding
- GO:0005624 membrane fraction
- GO:0005667 transcription factor complex
- GO:0005958 DNA-dependent protein kinase-DNA ligase 4 complex
- GO:0006266 DNA ligation
- GO:0006303 double-strand break repair via nonhomologous end joining
- GO:0006350 transcription
- GO:0008022 protein C-terminus binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0016481 negative regulation of transcription
- GO:0016563 transcription activator activity
- GO:0019047 provirus integration
- GO:0019059 initiation of viral infection
- GO:0043564 Ku70:Ku80 complex
- GO:0051575 5'-deoxyribose-5-phosphate lyase activity
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Ku70 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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