Ku70 antibody | 4C2-1A6

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Mouse anti Human Ku70:Preservative Free

Product Type
Monoclonal Antibody
Clone
4C2-1A6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA3127Z E IF P* WB 0.1 mg
Mouse anti Human Ku70 antibody, clone 4C2-1A6 recognizes human X-ray repair cross-complementing protein 6 (XRCC6), also known as Ku70, Lupus Ku autoantigen protein p70, CTC box-binding factor 75 kDa subunit or ATP-dependent DNA helicase 2 subunit 1. Ku70 is a 609 amino acid ~70 kDa ATP dependent helicase involved in chromosome translocation (UniProt: P12956).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1 - 610 of human Ku70 conjugated to glutathione S-transferase (GST).
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12956 Related reagents
Entrez Gene
XRCC6 Related reagents
GO Terms
GO:0000723 telomere maintenance
GO:0000783 nuclear telomere cap complex
GO:0003690 double-stranded DNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0005624 membrane fraction
GO:0005667 transcription factor complex
GO:0005958 DNA-dependent protein kinase-DNA ligase 4 complex
GO:0006266 DNA ligation
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0006350 transcription
GO:0008022 protein C-terminus binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016481 negative regulation of transcription
GO:0016563 transcription activator activity
GO:0019047 provirus integration
GO:0019059 initiation of viral infection
GO:0043564 Ku70:Ku80 complex
GO:0051575 5'-deoxyribose-5-phosphate lyase activity
Regulatory
For research purposes only

Applications of Ku70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 5 ug/ml
Immunofluorescence 5 20 ug/ml
Immunohistology - Paraffin 1 1 5 ug/ml
Western Blotting 1 5 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast cancer.
Western Blotting
Mouse anti Human Ku70 antibody, clone 4C2-1A6 detects a band of ~70 kDa in western blotting using lysates from either C32 cell lines or XRCC6-transfected 293T cell lines.

Product Specific References

References for Ku70 antibody

  1. Slupianek, A. et al. (2011) BCR/ABL Stimulates WRN to Promote Survival and Genomic Instability.
    Cancer Res. 71: 842-51.