KIND2 antibody | OTI25A4
Mouse anti KIND2
Mouse anti human KIND2 antibody recognizes fermitin family homolog 2, also known as FERMT2, PH domain-containing family C member 1, or pleckstrin homology domain containing, family C (with FERM domain) member 1. This antibody has been validated for western blotting using whole cell lysates.
KIND2 Antibody with Control Lysate
Mouse anti human KIND2 antibody recognizes fermitin family homolog 2, also known as FERMT2, PH domain-containing family C member 1, kindlin 2, mitogen inducible gene 2 protein or pleckstrin homology domain containing, family C (with FERM domain) member 1.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI25A4
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00399 | WB | 100 µl | ||||
| VMA00399KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human KIND2 antibody recognizes KIND2, also known as FERMT2, PH domain-containing family C member 1, fermitin family homolog 2, kindlin 2, mitogen inducible gene 2 protein and pleckstrin homology domain-containing family C member 1.
Mouse anti Human KIND2 antibody detects a band of 77 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human KIND2 antibody detects a band of 77 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti KIND2 detects a band of approximately 77 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full-length protein expressed in 293T cell transfected with human KIND2 expression vector
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q96AC1 Related reagents
- Entrez Gene
- FERMT2 Related reagents
- GO Terms
- GO:0001725 stress fiber
- GO:0030036 actin cytoskeleton organization
- GO:0007155 cell adhesion
- GO:0005829 cytosol
- GO:0005488 binding
- GO:0005925 focal adhesion
- GO:0005938 cell cortex
- GO:0008360 regulation of cell shape
- GO:0034329 cell junction assembly
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of KIND2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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