Ki67 antibody | AbD02531

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Human anti Ki67

Product Type
Monoclonal Antibody
Clone
AbD02531
Isotype
HuCAL/mouse IgG1 Fc
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA053 C E P* WB 0.1 mg
Human anti Ki67 antibody, clone AbD02531 recognizes the Ki67 cell-cycle associated protein. Ki67 is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki67 gene contains 15 exons. The Ki67 repeat region, within which there is a 22-amino acid Ki67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis reveals multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quiescent cells. Immunoblot analysis shows expression of 320 and 359 kDa proteins. Antisense oligonucleotides inhibit cellular proliferation in a dose-dependent manner, suggesting that Ki67 protein expression may be an absolute requirement for cell proliferation.

Within cells Ki67 is predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Macaque Expected from Sequence
Dog Expected from Sequence
Chimpanzee Expected from Sequence
Rhesus Monkey Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Chimeric human-mouse IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. It can be detected by anti mouse Fc specific secondary antibodies. This antibody is supplied liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Peptide derived from the human Ki67 protein, sequence GFKELFQTPG, coupled via a C-terminal cysteine to carrier proteins
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P46013 Related reagents
Entrez Gene
MKI67 Related reagents
GO Terms
GO:0005524 ATP binding
GO:0005730 nucleolus
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Ki67 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2.0 ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin 1 0.2 ug/ml 1.0 ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Histology Positive Control Tissue
Human tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 0.5 ml E WB
Histar Detection System STAR3000A 50 Tests C P

Product Specific References

References for Ki67 antibody

  1. Jarutat, T. et al. (2006) Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library.
    Biol. Chem. 387: 995-1003.
  2. Liu, S.K. et al. (2011) Delta-like ligand 4-notch blockade and tumor radiation response.
    J Natl Cancer Inst. 103 (23): 1778-98.
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