KBNB1 antibody | AB04/1A8

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Mouse anti KBNB1

Product Type
PrecisionAb Monoclonal
Clone
AB04/1A8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00612
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human KBNB1 antibody recognizes KPNB1, also known as importin Beta 1, PTAC97, IPO1, IMB1 or NTF97.

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Feb 2013).

Mouse anti Human KBNB1 antibody detects a band of 97 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti KBNB1 antibody detects a band of approximately 97 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human KBNB1 (aa 1-155)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q14974 Related reagents
Entrez Gene
KPNB1 Related reagents
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0005829 cytosol
GO:0005643 nuclear pore
GO:0005654 nucleoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006607 NLS-bearing substrate import into nucleus
GO:0008139 nuclear localization sequence binding
GO:0008270 zinc ion binding
GO:0019058 viral infectious cycle
GO:0019904 protein domain specific binding
GO:0046796 viral genome transport in host cell
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of KBNB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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