KBNB1 antibody | AB04/1A8

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Anti KBNB1 Antibody, clone AB04/1A8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti KBNB1 Antibody, clone AB04/1A8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with KBNB1 antibody (**VMA00612**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to KBNB1 (molecular weight 97 kDa).

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Mouse anti KBNB1

Product Type: PrecisionAb Monoclonal
Clone: AB04/1A8
Isotype: IgG2b
Format: Purified
Specificity: KBNB1

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VMA00612 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human KBNB1 antibody recognizes KPNB1, also known as importin Beta 1, PTAC97, IPO1, IMB1 or NTF97.

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Feb 2013).

Mouse anti Human KBNB1 antibody detects a band of 97 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti KBNB1 antibody detects a band of approximately 97 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human KBNB1 (aa 1-155)
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q14974
Entrez Gene: KPNB1
GO Terms: GO:0000060 protein import into nucleus, translocation; GO:0005829 cytosol; GO:0005643 nuclear pore; GO:0005654 nucleoplasm; GO:0006309 DNA fragmentation involved in apoptotic nuclear change; GO:0006607 NLS-bearing substrate import into nucleus; GO:0008139 nuclear localization sequence binding; GO:0008270 zinc ion binding; GO:0019058 viral infectious cycle; View More GO Terms; GO:0019904 protein domain specific binding; GO:0046796 viral genome transport in host cell