Kars antibody | AB01/3F8

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Mouse anti Kars

Product Type
PrecisionAb Monoclonal
Clone
AB01/3F8
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00877
Datasheet
IP WB
SDS
100 µl loader

Mouse anti KARS antibody recognizes lysine--tRNA ligase, also known as lysyl-tRNA synthetase and LysRS.

tRNA synthetases are responsible for setting the genetic code during the first step of protein synthesis by specifically linking a single amino acid to a set of tRNA isoacceptors (Pang et al. 2014). The KARS gene encodes lysyl-tRNA synthetase, which catalyzes the attachment of a specific amino acid to its cognate tRNA in both the cytoplasm and mitochondria (Ardissone et al. 2018). Mutations in KARS have been asociated with various pathologies including autosomal recessive nonsyndromic hearing loss, congenital visual impairment and progressive microcephaly (Verrigni et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti KARS antibody detects a band of approximately 74 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-597 of human KARS
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15046
Entrez Gene
KARS
GO Terms
GO:0005886 plasma membrane
GO:0000049 tRNA binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0004824 lysine-tRNA ligase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0005759 mitochondrial matrix
GO:0006430 lysyl-tRNA aminoacylation
GO:0008033 tRNA processing
GO:0044419 interspecies interaction between organisms
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Kars antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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