Jnk (pThr183/pTyr185) antibody | D04-7G6
Mouse anti JNK (pThr183/pTyr185) antibody, clone D04-7G6 recognizes the c-Jun N-terminal kinase (JNK), when phosphorylated at threonine 183 and tyrosine 185. Phosphorylation of these residues is associated with the activation of JNK following exposure to enviromental stress (e.g. UV).
JNKs are members of the mitogen activated protein kinase (MAPK) family which constitute several signalling pathways. In this signalling cascade extracellular stimuli activate small GTAPases (of the Rho family) which in turn acitvate MAP2K4/MKK4 and MAP2K7/MKK7. These kinases then in turn activate JNK. JNK then interacts with a variety of downstream targets including other signalling molcules and transcription factors (Sehgal & Ram, 2013).
JNKs are members of the mitogen activated protein kinase (MAPK) family which constitute several signalling pathways. In this signalling cascade extracellular stimuli activate small GTAPases (of the Rho family) which in turn acitvate MAP2K4/MKK4 and MAP2K7/MKK7. These kinases then in turn activate JNK. JNK then interacts with a variety of downstream targets including other signalling molcules and transcription factors (Sehgal & Ram, 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to residues surrounding threonine 183 and tyrosine 185 of human JNK
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BLAB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Jnk (pThr183/pTyr185) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting 1 | 1/1000 |
- 1Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Further Reading
-
Sehgal, V. & Ram, P.T. (2013) Network Motifs in JNK Signaling
Genes Cancer 4: 409-13
