JAB1 antibody

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Mouse anti JAB1

Mouse anti human JAB1 antibody recognizes the COP9 signalosome complex subunit 5, also known as 38 kDa Mov34 homolog, COP9 constitutive photomorphogenic homolog subunit 5, or signalosome subunit 5. This antibody has been extensively validated for western blotting using whole cell lysates.

JAB1 Antibody with Control Lysate

Mouse anti human JAB1 antibody recognizes the COP9 signalosome complex subunit 5, also known as 38 kDa Mov34 homolog, COP9 constitutive photomorphogenic homolog subunit 5, jun activation domain-binding protein 1 or signalosome subunit 5.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2b
Format: Purified

Product Code	Applications	Pack Size
VMA00414	WB	100 µl
VMA00414KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human JAB1 antibody recognizes JAB1, also known as 38 kDa Mov34 homolog, COP9 constitutive photomorphogenic homolog subunit 5, COP9 signalosome complex subunit 5, COPS5, jun activation domain-binding protein 1 or signalosome subunit 5.

The protein encoded by the COPS5 gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be a coactivator that increases the specificity of JUN/AP1 transcription factors (provided by RefSeq, Jul 2008).

Mouse anti Human JAB1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti JAB1 detects a band of approximately 36 kDa in Jurkat cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

E.coli-derived recombinant human JAB1

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: Q92905 Related reagents
Entrez Gene: COPS5 Related reagents
GO Terms: GO:0005515 protein binding; GO:0000338 protein deneddylation; GO:0003713 transcription coactivator activity; GO:0003743 translation initiation factor activity; GO:0008237 metallopeptidase activity; GO:0046872 metal ion binding; GO:0005852 eukaryotic translation initiation factor 3 complex; GO:0006366 transcription from RNA polymerase II promoter; GO:0008180 signalosome
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of JAB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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