J Chain antibody | Mc19-9

Mouse anti Human J Chain
- Product Type
- Monoclonal Antibody
- Clone
- Mc19-9
- Isotype
- IgG1
- Specificity
- J Chain
Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques Mouse anti Human J chain antibody, clone Mc19-9 detects J chain in IgM only under reducing conditions. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human J Chain.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
5ug/ml | |
Immunohistology - Frozen | ![]() |
1/200 | |
Immunoprecipitation | ![]() |
20ug/ml | |
Western Blotting | ![]() |
References for J Chain antibody
-
Arnold, J.N. et al. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin.
J Biol Chem. 280 (32): 29080-7. -
Almogren, A. et al. (2006) Purification, properties and extended solution structure of the complex formed between human immunoglobulin A1 and human serum albumin by scattering and ultracentrifugation.
J Mol Biol. 356 (2): 413-31.
- RRID
- AB_2121623
MCA693



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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