J Chain antibody | Mc19-9
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Mouse anti Human J Chain
- Product Type
- Monoclonal Antibody
- Clone
- Mc19-9
- Isotype
- IgG1
- Specificity
- J Chain
Antibody Quality and 
Unrivaled 
| Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques Mouse anti Human J chain antibody, clone Mc19-9 detects J chain in IgM only under reducing conditions. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human J Chain.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml | |
| Immunohistology - Frozen | |
1/200 | |
| Immunoprecipitation | |
20ug/ml | |
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for J Chain antibody
-
Arnold, J.N. et al. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin.
J Biol Chem. 280 (32): 29080-7. -
Almogren, A. et al. (2006) Purification, properties and extended solution structure of the complex formed between human immunoglobulin A1 and human serum albumin by scattering and ultracentrifugation.
J Mol Biol. 356 (2): 413-31.
- RRID
- AB_2121623
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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