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J Chain antibody | Mc19-9

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Mouse anti Human J Chain

Product Type
Monoclonal Antibody
Clone
Mc19-9
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA693
Datasheet
C E IP WB
MSDS
0.2 mg loader

Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen.

Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques Mouse anti Human J chain antibody, clone Mc19-9 detects J chain in IgM only under reducing conditions.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human J Chain.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of J Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml
Immunohistology - Frozen 1/200
Immunoprecipitation 20ug/ml
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for J Chain antibody

  1. Arnold, J.N. et al. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin.
    J Biol Chem. 280 (32): 29080-7.
  2. Almogren, A. et al. (2006) Purification, properties and extended solution structure of the complex formed between human immunoglobulin A1 and human serum albumin by scattering and ultracentrifugation.
    J Mol Biol. 356 (2): 413-31.
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