J Chain antibody | Mc19-9
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Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen.
Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques Mouse anti Human J chain antibody, clone Mc19-9 detects J chain in IgM only under reducing conditions.
Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques Mouse anti Human J chain antibody, clone Mc19-9 detects J chain in IgM only under reducing conditions.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human J Chain.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of J Chain antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 5ug/ml | ||
| Immunohistology - Frozen | 1/200 | ||
| Immunoprecipitation | 20ug/ml | ||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for J Chain antibody
-
Arnold, J.N. et al. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin.
J Biol Chem. 280 (32): 29080-7. -
Almogren, A. et al. (2006) Purification, properties and extended solution structure of the complex formed between human immunoglobulin A1 and human serum albumin by scattering and ultracentrifugation.
J Mol Biol. 356 (2): 413-31.
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