Isocitrate Dehydrogenase 3A antibody | OTI5D2

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Mouse anti Isocitrate Dehydrogenase 3A

Product Type
PrecisionAb Monoclonal
Clone
OTI5D2
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00722
Datasheet
WB
SDS
100 µl loader

Mouse anti Human isocitrate dehydrogenase 3A antibody recognizes the isocitrate dehydrogenase 3A, also known as H-IDH alpha, NAD(+)-specific ICDH subunit alpha, NAD(H)-specific isocitrate dehydrogenase alpha subunit and isocitrate dehydrogenase (NAD+) alpha chain.

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by IDH3A is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase (provided by RefSeq, Jul 2008).

Mouse anti Human isocitrate dehydrogenase 3A antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti isocitrate dehydrogenase 3A detects a band of approximately 39 kDa in Raji cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
HEK293-dervied full length recombinant protein of human isocitrate dehydrogenase 3A

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P50213
Entrez Gene
IDH3A
GO Terms
GO:0000287 magnesium ion binding
GO:0004449 isocitrate dehydrogenase (NAD+) activity
GO:0005759 mitochondrial matrix
GO:0005975 carbohydrate metabolic process
GO:0006099 tricarboxylic acid cycle
GO:0051287 NAD binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Isocitrate Dehydrogenase 3A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml