Isocitrate Dehydrogenase 1 antibody | OTI2H9
Mouse anti Isocitrate Dehydrogenase 1
Isocitrate dehydrogenase 1 antibody recognizes the isocitrate dehydrogenase [NADP] cytoplasmic protein, also known as NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, epididymis luminal protein 216, or epididymis secretory protein Li 26.
- Product Type
- PrecisionAb™ Monoclonal
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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. Encoded by IDH1 gene, isocitrate dehydrogenase 1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for IDH1 (provided by RefSeq, Sep 2013).
Mouse anti Human isocitrate dehydrogenase 1 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti isocitrate dehydrogenase 1 detects a band of approximately 46 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Full-length protein expressed in 293T cell transfected with human isocitrate dehydrogenase 1 expression vector
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- O75874 Related reagents
- Entrez Gene
- IDH1 Related reagents
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004450 isocitrate dehydrogenase (NADP+) activity
- GO:0005829 cytosol
- GO:0005782 peroxisomal matrix
- GO:0006097 glyoxylate cycle
- GO:0006099 tricarboxylic acid cycle
- GO:0006103 2-oxoglutarate metabolic process
- GO:0006102 isocitrate metabolic process
- GO:0006740 NADPH regeneration
- GO:0042803 protein homodimerization activity
- GO:0044255 cellular lipid metabolic process
- GO:0051287 NAD binding
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of Isocitrate Dehydrogenase 1 antibody
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PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||2 ml||WB|
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