Isocitrate Dehydrogenase 1 antibody | OTI2H9
Mouse anti Isocitrate Dehydrogenase 1
Isocitrate dehydrogenase 1 antibody recognizes the isocitrate dehydrogenase [NADP] cytoplasmic protein, also known as NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, epididymis luminal protein 216, or epididymis secretory protein Li 26.
Isocitrate Dehydrogenase 1 Antibody with Control Lysate
Isocitrate dehydrogenase 1 antibody recognizes the isocitrate dehydrogenase [NADP] cytoplasmic protein, also known as NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, or epididymis luminal protein 216.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2H9
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00143 | WB | 100 µl | ||||
| VMA00143KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human isocitrate dehydrogenase 1 antibody recognizes isocitrate dehydrogenase 1, also known as NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, epididymis luminal protein 216, epididymis secretory protein Li 26, isocitrate dehydrogenase [NADP] cytoplasmic and oxalosuccinate decarboxylase.
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. Encoded by IDH1 gene, isocitrate dehydrogenase 1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for IDH1 (provided by RefSeq, Sep 2013).
Mouse anti Human isocitrate dehydrogenase 1 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. Encoded by IDH1 gene, isocitrate dehydrogenase 1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for IDH1 (provided by RefSeq, Sep 2013).
Mouse anti Human isocitrate dehydrogenase 1 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti isocitrate dehydrogenase 1 detects a band of approximately 46 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full-length protein expressed in 293T cell transfected with human isocitrate dehydrogenase 1 expression vector
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O75874 Related reagents
- Entrez Gene
- IDH1 Related reagents
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004450 isocitrate dehydrogenase (NADP+) activity
- GO:0005829 cytosol
- GO:0005782 peroxisomal matrix
- GO:0006097 glyoxylate cycle
- GO:0006099 tricarboxylic acid cycle
- GO:0006103 2-oxoglutarate metabolic process
- GO:0006102 isocitrate metabolic process
- GO:0006740 NADPH regeneration
- GO:0042803 protein homodimerization activity
- GO:0044255 cellular lipid metabolic process
- GO:0051287 NAD binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Isocitrate Dehydrogenase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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