IRF8 antibody | CD01/4H2

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Mouse anti IRF8

Product Type
PrecisionAb Monoclonal
Clone
CD01/4H2
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00855
Datasheet
WB
SDS
100 µl loader

Mouse anti IRF8 antibody recognizes interferon regulatory factor 8, also known as ICSBP.

IRF8 is an interferon regulatory factor, a group of proteins with essential roles in the innate and adaptive immune system. IRF8 is a transcription factor, and an important regulator of the function of macrophages, B-cells and dendritic cells, and of differentiation of Th9, Th17, and Treg cells (Humblin et al. 2017). IRF8 also facilitates the proliferative burst of natural killer cells during acute viral infection (Adams et al. 2018). IRF8 appears to act as a tumor suppressor in various cancers, including myeloid leukemia (Gaillard et al. 2018) and breast cancers, with IRF8 downregulated in breast cancers and restoration of gene function suppressing cell proliferation and other processes (Luo et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti IRF8 antibody detects a band of approximately 50 kDa in Ramos cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-426 of human IRF8
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q02556
Entrez Gene
IRF8
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0003677 DNA binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005634 nucleus
GO:0006955 immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of IRF8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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