IRF8 antibody | CD01/4H2
Mouse anti IRF8 antibody recognizes interferon regulatory factor 8, also known as ICSBP.
IRF8 is an interferon regulatory factor, a group of proteins with essential roles in the innate and adaptive immune system. IRF8 is a transcription factor, and an important regulator of the function of macrophages, B-cells and dendritic cells, and of differentiation of Th9, Th17, and Treg cells (Humblin et al. 2017). IRF8 also facilitates the proliferative burst of natural killer cells during acute viral infection (Adams et al. 2018). IRF8 appears to act as a tumor suppressor in various cancers, including myeloid leukemia (Gaillard et al. 2018) and breast cancers, with IRF8 downregulated in breast cancers and restoration of gene function suppressing cell proliferation and other processes (Luo et al. 2017).
IRF8 is an interferon regulatory factor, a group of proteins with essential roles in the innate and adaptive immune system. IRF8 is a transcription factor, and an important regulator of the function of macrophages, B-cells and dendritic cells, and of differentiation of Th9, Th17, and Treg cells (Humblin et al. 2017). IRF8 also facilitates the proliferative burst of natural killer cells during acute viral infection (Adams et al. 2018). IRF8 appears to act as a tumor suppressor in various cancers, including myeloid leukemia (Gaillard et al. 2018) and breast cancers, with IRF8 downregulated in breast cancers and restoration of gene function suppressing cell proliferation and other processes (Luo et al. 2017).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti IRF8 antibody detects a band of approximately 50 kDa in Ramos cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-426 of human IRF8
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q02556
- Entrez Gene
- IRF8
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0003677 DNA binding
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005634 nucleus
- GO:0006955 immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of IRF8 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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