IRF8 antibody | CD01/4H2

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Anti IRF8 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti IRF8 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with IRF8 antibody (1/1,000, **VMA00855**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to IRF8 (molecular weight 50 kDa)

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Mouse anti IRF8

Product Type: PrecisionAb Monoclonal
Clone: CD01/4H2
Isotype: IgG2a
Format: Purified
Specificity: IRF8

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VMA00855 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti IRF8 antibody recognizes interferon regulatory factor 8, also known as ICSBP.

IRF8 is an interferon regulatory factor, a group of proteins with essential roles in the innate and adaptive immune system. IRF8 is a transcription factor, and an important regulator of the function of macrophages, B-cells and dendritic cells, and of differentiation of Th9, Th17, and Treg cells (Humblin et al. 2017). IRF8 also facilitates the proliferative burst of natural killer cells during acute viral infection (Adams et al. 2018). IRF8 appears to act as a tumor suppressor in various cancers, including myeloid leukemia (Gaillard et al. 2018) and breast cancers, with IRF8 downregulated in breast cancers and restoration of gene function suppressing cell proliferation and other processes (Luo et al. 2017).

Target Species: Human
Western Blotting: Mouse anti IRF8 antibody detects a band of approximately 50 kDa in Ramos cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-426 of human IRF8
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q02556
Entrez Gene: IRF8
GO Terms: GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0003677 DNA binding; GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0005634 nucleus; GO:0006955 immune response; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060337 type I interferon-mediated signaling pathway