Involucrin antibody | SY5
Involucrin expression is seen in the normal granular cells of the epidermis associated with the plasma membrane during the early phase of cornified envelope maturation (Ishida-Yamamoto et al. 1996) and expression is increased in psoriatic epidermis with abnormal cornified envelope maturation (Ishida-Yamamoto and Iizuka 1995). Involucrin staining is also maintained, associated with the cornified epidermis in epidermolytic hyperkeratosis, a condition associated with mutations to mutations in genes codong for cytokeratins 1 and 10 (Ishida-Yamamoto et al. 1995).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Monkey Dog Gorilla - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified involucrin from human keratinocytes.
- Approx. Protein Concentrations
- 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mise were fused with cells from the P3X63Ag8 murine myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07476
- Entrez Gene
- IVL
- GO Terms
- GO:0001533 cornified envelope
- GO:0030674 protein binding, bridging
- GO:0005198 structural molecule activity
- GO:0005737 cytoplasm
- GO:0010224 response to UV-B
- GO:0018153 isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine
- GO:0031424 keratinization
- Regulatory
- For research purposes only
Applications of Involucrin antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting |
- Histology Positive Control Tissue
- MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Secondary Antibodies Available
Product Specific References
References for Involucrin antibody
-
Hudson, D.L. et al. (1992) Characterization of eight monoclonal antibodies to involucrin.
Hybridoma . 3: 367-79. -
Ishida-Yamamoto, A. et al. (1997) Involucrin and SPRR Are Synthesized Sequentially in Differentiating Cultured Epidermal Cells.
J Invest Dermatol. 108: 12-6. -
Caldelari, R. et al. (2000) Long-term culture of murine epidermal keratinocytes.
J Invest Dermatol. 114: 1064-5. -
Ishida-Yamamoto, A. et al. (1996) Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis.
J Histochem Cytochem. 44: 167-75. -
Janes,S.M. and Watt, F.M. (2004) Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis.
J Cell Biol. 166: 419-31. -
Michael, M. et al. (2014) BPAG1-e restricts keratinocyte migration through control of adhesion stability.
J Invest Dermatol. 134: 773-82. -
Hanada, T. et al. (2014) Keratinization induced by air exposure in the reconstructed human epidermal model: An in vitro model of a cultured epithelial autograft
J Biosci Bioeng. pii: S1389-1723(14)00071-1. -
Sawant S et al. (2016) Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression.
PLoS One. 11 (8): e0160615. -
Srivastava, S.S. et al. (2018) Keratin 5/14‑mediated cell differentiation and transformation are regulated by TAp63 and Notch‑1 in oral squamous cell carcinoma‑derived cells.
Oncol Rep. Mar 06 [Epub ahead of print].
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