Involucrin antibody | SY5

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Anti Human Involucrin Antibody, clone SY5 gallery image 1 — **Published customer image:**
Mouse anti Human involucrin antibody, clone SY5 used for the identification of involucrin expressing cells in dysplasic tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
**Image caption:**
Cell proliferation and differentiation staining pattern in in-vitro grown tissues.
Immunohistochemical staining for cell proliferation and differentiation specific proteins in Fib+ and Fib- OCs reconstructed from normal, dysplastic and malignant tongue tissues along with their respective native tissues: PCNA nuclear staining was seen in the basal proliferating cells of normal- native tissue, Fib+ and Fib- OCs (a, b, c) while cytoplasmic involucrin staining was seen in the upper differentiated cells of normal- native tissue, Fib+ and Fib- OCs (d, e, f). PCNA nuclear staining was seen in the basal as well as supra basal cells of dysplastic-native tissue, Fib+ and Fib- OCs (g, h, i) while cytoplasmic involucrin staining was seen in the native dysplastic tissue but not in Fib+ and Fib- OCs (j, k, l). PCNA staining was observed throughout the epithelium of malignant-native tissue, Fib+ and Fib- OCs (m, n, o), while involucrin staining was seen in the differentiated cells of malignant-native tissue but not in the Fib+ and Fib- OCs (p, q, r). The experiments were performed at least three times.

From: Sawant S, Dongre H, Singh AK, Joshi S, Costea DE, Mahadik S, *et al*. (2016)
Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression.
PLoS ONE 11(8): e0160615.

Mouse anti Human Involucrin

Product Type: Monoclonal Antibody
Clone: SY5
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
5390-9950	C E IP P WB			0.1 mg

Mouse anti human involucrin antibody, clone SY5 recognizes human involucrin, a 585 amino acid soluble protein encoded by the IVL gene expressed in epidermal keratinocytes as they tend towards terminal differentiation. Involucrin is produced in the stratum spinosum initially in the cell cytosol from where it moves to the cell membrane, associates with transglutaminase and becomes cross-linked to membrane proteins, offering structural support to the cell and forming a stable envelope below the plasma membrane. Mouse anti human involucrin antibody, clone SY5 recognizes an epitope between residues 421-568 of human involucrin.

Involucrin expression is seen in the normal granular cells of the epidermis associated with the plasma membrane during the early phase of cornified envelope maturation (Ishida-Yamamoto et al. 1996) and expression is increased in psoriatic epidermis with abnormal cornified envelope maturation (Ishida-Yamamoto and Iizuka 1995). Involucrin staining is also maintained, associated with the cornified epidermis in epidermolytic hyperkeratosis, a condition associated with mutations to mutations in genes codong for cytokeratins 1 and 10 (Ishida-Yamamoto et al. 1995).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Pig
Monkey
Dog
Gorilla

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Purified involucrin from human keratinocytes.

Approx. Protein Concentrations

0.5 mg/ml

Fusion Partners

Spleen cells from immunized Balb/c mise were fused with cells from the P3X63Ag8 murine myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P07476 Related reagents
Entrez Gene: IVL Related reagents
GO Terms: GO:0001533 cornified envelope; GO:0030674 protein binding, bridging; GO:0005198 structural molecule activity; GO:0005737 cytoplasm; GO:0010224 response to UV-B; GO:0018153 isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; GO:0031424 keratinization
Regulatory: For research purposes only

Applications of Involucrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Flow Cytometry

Product Specific References

References for Involucrin antibody

Hudson, D.L. et al. (1992) Characterization of eight monoclonal antibodies to involucrin.
Hybridoma . 3: 367-79.
Ishida-Yamamoto, A. et al. (1997) Involucrin and SPRR Are Synthesized Sequentially in Differentiating Cultured Epidermal Cells.
J Invest Dermatol. 108: 12-6.
Caldelari, R. et al. (2000) Long-term culture of murine epidermal keratinocytes.
J Invest Dermatol. 114: 1064-5.
Ishida-Yamamoto, A. et al. (1996) Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis.
J Histochem Cytochem. 44: 167-75.
Janes,S.M. and Watt, F.M. (2004) Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis.
J Cell Biol. 166: 419-31.
Michael, M. et al. (2014) BPAG1-e restricts keratinocyte migration through control of adhesion stability.
J Invest Dermatol. 134: 773-82.
Hanada, T. et al. (2014) Keratinization induced by air exposure in the reconstructed human epidermal model: An in vitro model of a cultured epithelial autograft
J Biosci Bioeng. pii: S1389-1723(14)00071-1.
Sawant S et al. (2016) Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression.
PLoS One. 11 (8): e0160615.
Srivastava, S.S. et al. (2018) Keratin 5/14‑mediated cell differentiation and transformation are regulated by TAp63 and Notch‑1 in oral squamous cell carcinoma‑derived cells.
Oncol Rep. Mar 06 [Epub ahead of print].

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