Inhibin Alpha antibody | AbD05070

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Human anti Human Inhibin Alpha

Product Type
Monoclonal Antibody
Clone
AbD05070
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA070 C E P* 0.1 mg
Human anti Human Inhibin alpha antibody, clone AbD05070 recognizes the 32 kDa alpha subunit of human inhibin.

Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumors, placental and gestational trophoblastic lesions and sex cord stromal tumors.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Peptide comprising the first 29 amino acids of the mature inhibin alpha chain (amino acids 233-262)
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P05111 Related reagents
Entrez Gene
INHA Related reagents
GO Terms
GO:0001501 skeletal system development
GO:0001541 ovarian follicle development
GO:0004871 signal transducer activity
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0006917 induction of apoptosis
GO:0007050 cell cycle arrest
GO:0007166 cell surface receptor linked signaling pathway
GO:0007267 cell-cell signaling
GO:0007399 nervous system development
GO:0008083 growth factor activity
GO:0009605 response to external stimulus
GO:0046881 positive regulation of follicle-stimulating hormone secretion
GO:0046882 negative regulation of follicle-stimulating hormone secretion
GO:0030218 erythrocyte differentiation
GO:0034673 inhibin-betaglycan-ActRII complex
GO:0042127 regulation of cell proliferation
GO:0042326 negative regulation of phosphorylation
GO:0042541 hemoglobin biosynthetic process
GO:0043512 inhibin A complex
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0045578 negative regulation of B cell differentiation
GO:0045650 negative regulation of macrophage differentiation
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Inhibin Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/500
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 0.5 ml E WB
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB