ING2 antibody | OTI3B11
Mouse anti ING2
ING2 antibody recognizes inhibitor of growth protein 2, also known as ING1Lp, or inhibitor of growth 1-like protein.
ING2 Antibody with Control Lysate
ING2 antibody recognizes inhibitor of growth protein 2, also known as ING1Lp, or inhibitor of growth 1-like protein.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3B11
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00741 | WB | 100 µl | ||||
| VMA00741KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ING2 antibody recognizes the inhibitor of growth protein 2, also known as ING1Lp and inhibitor of growth 1-like protein.
ING2 is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis (provided by RefSeq, Jul 2008).
Mouse anti Human ING2 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
ING2 is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis (provided by RefSeq, Jul 2008).
Mouse anti Human ING2 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti ING2 detects a band of approximately 36 kDa in HeLa cell lysates
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Immunogen
- E. coli-derived full length human recombinant protein of human ING2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9H160 Related reagents
- Entrez Gene
- ING2 Related reagents
- GO Terms
- GO:0003677 DNA binding
- GO:0003682 chromatin binding
- GO:0007165 signal transduction
- GO:0006350 transcription
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0008270 zinc ion binding
- GO:0016563 transcription activator activity
- GO:0016568 chromatin modification
- GO:0016580 Sin3 complex
- GO:0016602 CCAAT-binding factor complex
- GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
- GO:0032403 protein complex binding
- GO:0040008 regulation of growth
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ING2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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