ING2 antibody | OTI3B11

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Mouse anti ING2

ING2 antibody recognizes inhibitor of growth protein 2, also known as ING1Lp, or inhibitor of growth 1-like protein.

ING2 Antibody with Control Lysate

ING2 antibody recognizes inhibitor of growth protein 2, also known as ING1Lp, or inhibitor of growth 1-like protein.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3B11
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00741 WB 100 µl
VMA00741KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ING2 antibody recognizes the inhibitor of growth protein 2, also known as ING1Lp and inhibitor of growth 1-like protein.

ING2 is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis (provided by RefSeq, Jul 2008).

Mouse anti Human ING2 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti ING2 detects a band of approximately 36 kDa in HeLa cell lysates
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
E. coli-derived full length human recombinant protein of human ING2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9H160 Related reagents
Entrez Gene
ING2 Related reagents
GO Terms
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0007165 signal transduction
GO:0006350 transcription
GO:0006355 regulation of transcription, DNA-dependent
GO:0008270 zinc ion binding
GO:0016563 transcription activator activity
GO:0016568 chromatin modification
GO:0016580 Sin3 complex
GO:0016602 CCAAT-binding factor complex
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0032403 protein complex binding
GO:0040008 regulation of growth
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ING2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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