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Indoleamine 2,3-Dioxygenase antibody | 4D2

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Anti Human Indoleamine 2,3-Dioxygenase Antibody, clone 4D2 thumbnail image 1 Anti Human Indoleamine 2,3-Dioxygenase Antibody, clone 4D2 thumbnail image 2
Anti Human Indoleamine 2,3-Dioxygenase Antibody, clone 4D2 gallery image 1
Western Blot analysis of indolamine 2;3-dioxygenase expression in HeLa cell lysate using Mouse anti Human indolamine 2;3-dioxygenase antibody (MCA5433Z)
Anti Human Indoleamine 2,3-Dioxygenase Antibody, clone 4D2 gallery image 2
Staining of paraffin-embedded human spleen with Mouse anti Human indolamine 2;3-dioxygenase antibody (MCA5433Z)

Mouse anti Human Indoleamine 2,3-Dioxygenase:Preservative Free

Product Type
Monoclonal Antibody
Clone
4D2
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA5433Z
Datasheet
P* WB
MSDS
0.1 mg loader

Mouse anti Human indoleamine 2,5-dioxygenase antibody, clone 4D2 recognizes human Indoleamine 2,3-dioxygenase 1, also known IDO-1. IDO-1 is a 403 amino acid ~50 kDa enzyme involved in tryptophan catabolism. IDO-1 is expressed in lymphoid organ dendritic cells and in some epithelial cells. It is not expressed in most tissues but expression is usually inducible by interferon γ (Taylor and Feng 1991).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1-404 of human Indoleamine 2,3-dioxygenase.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P14902 Related reagents
Entrez Gene
IDO1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005829 cytosol
GO:0004833 tryptophan 2,3-dioxygenase activity
GO:0055114 oxidation-reduction process
GO:0006569 tryptophan catabolic process
GO:0007565 female pregnancy
GO:0020037 heme binding
GO:0033754 indoleamine 2,3-dioxygenase activity
Regulatory
For research purposes only

Applications of Indoleamine 2,3-Dioxygenase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting 0.1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Human Spleen
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Indoleamine 2,3-Dioxygenase antibody

  1. Garraway, L. et al. (2016) Kynurenine Pathway Biomarkers Predictive of Anti-Immune Checkpoint Inhibitor Response.
    Publication number US20160299146 A1
  2. Brocard, A. et al. (2017) Clinical, Genetic and Innate Immunity Characteristics of Melanoma in Organ Transplant Recipients.
    Acta Derm Venereol. 97 (4): 483-8.
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