IL-9 antibody | MH9A4

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Mouse anti Human Interleukin-9

Mouse anti Human Interleukin-9:RPE

Product Type
Monoclonal Antibody
Clone
MH9A4
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA6074GA
Datasheet
E
MSDS
0.1 mg loader

MCA6074PE
Datasheet
F*
MSDS
100 Tests/0.5ml loader

Mouse anti Human interleukin-9 antibody, clone MH9A4 recognizes interleukin-9 (IL-9), also known as cytokine P40 and T cell growth factor P40.

IL-9 is a 14 kDa pro-inflammatory cytokine, secreted predominantly by T cells. Initally associated with the Th2 phenotype, IL-9 is now known to be secreted by other Th subsets including Th17. Through its interaction with the interleukin-9 receptor, and the downstream activation of STAT3, MAPK and PI3K pathways, IL-9 stimulates immune cell proliferation and inhibits apoptosis. IL-9 has long been recognized as an important mediator of asthma and the allergic response.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Baculovirus-expressed recombinant human interleukin-9
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P15248 Related reagents
Entrez Gene
IL9 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0045407 positive regulation of interleukin-5 biosynthetic process
Regulatory
For research purposes only

Applications of IL-9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 ug/ml 4 ug/ml
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests loader

Product Specific References

References for IL-9 antibody

  1. Pang, N. et al. (2018) TGF-β/Smad signaling pathway positively up-regulates the differentiation of Interleukin-9-producing CD4+T cells in human Echinococcus granulosus infection.
    J Infect. Jan 29 [Epub ahead of print].