IL-7 antibody | 2D8-24-25

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Mouse anti Human Interleukin-7:Preservative Free

Product Type
Monoclonal Antibody
Clone
2D8-24-25
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6336Z
Datasheet
E WB
SDS
0.5 mg loader

Mouse anti Human interleukin-7 antibody, clone 2D8-24-25, recognizes interleukin-7 (IL-7) a cytokine that plays roles in the early development of B and T cells. It is also required by mature T cells for survival, proliferation and many effector functions during infection and tumor infiltration (Sheikh & Abraham 2019). IL-7 elicits its biological functions through IL-7 receptor α. Upon ligand binding to IL-7 receptor α, the JAK and STAT signaling pathways are activated to subsequently activate downstream signaling pathways to initiate target gene transcription (Kittipatarin & Khaled 2007, Nguyen et al. 2017).

IL-7 is mainly produced by stromal cells in the bone marrow and thymus under steady states. However, IL-7 is also present at low levels in the small intestine, lungs, liver and skin where it can modulate T cell responses (Sheikh & Abraham 2019).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
E. coli derived recombinant human interleukin-7
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 1 EU/μg

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
6 months from date of despatch

More Information

UniProt
P13232
Entrez Gene
IL7
GO Terms
GO:0005125 cytokine activity
GO:0005139 interleukin-7 receptor binding
GO:0005615 extracellular space
GO:0006959 humoral immune response
GO:0007267 cell-cell signaling
GO:0009887 organ morphogenesis
GO:0008083 growth factor activity
GO:0030890 positive regulation of B cell proliferation
GO:0045453 bone resorption
GO:0045582 positive regulation of T cell differentiation
Regulatory
For research purposes only

Applications of IL-7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml 2.0 ug/ml
Western Blotting 0.25 ug/ml 0.5 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

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