Rat anti Human interleukin-6 antibody, clone MQ2-13A5
recognizes human interleukin 6, also known as B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor or Interferon beta-2. Interleukin-6 is a 183 amino acid ~24 kDa cytokine with a wide range of biological activities. a potent inducer of the acute phase response, acts as a lymphoid cell growth factor acting on B cells, T cells and hybridoma cells.
Variations in the interleukin-6 gene modulates susceptibility to onset of juvenile systemic rheumatoid arthritis (RASJ
) an inflammatory disorder with early onset and featuring lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis (Fishman et al. 1998
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- COS-7-expressed recombinant human IL-6
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
hepatic immune response
neutrophil mediated immunity
positive regulation of acute inflammatory response
positive regulation of leukocyte chemotaxis
interleukin-6 receptor binding
interleukin-6 receptor complex
humoral immune response
growth factor activity
defense response to Gram-positive bacterium
defense response to Gram-negative bacterium
negative regulation of cell proliferation
regulation of vascular endothelial growth factor production
negative regulation of lipid storage
positive regulation of translation
endocrine pancreas development
neuron projection development
response to peptidoglycan
positive regulation of chemokine production
positive regulation of interleukin-6 production
negative regulation of collagen biosynthetic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of apoptosis
positive regulation of gene-specific transcription
positive regulation of MAPKKK cascade
negative regulation of chemokine biosynthetic process
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
regulation of angiogenesis
positive regulation of anti-apoptosis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of B cell activation
positive regulation of immunoglobulin secretion
positive regulation of transcription factor activity
response to glucocorticoid stimulus
defense response to virus
interleukin-6-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-6 antibody
|Flow Cytometry 1
|Western Blotting 2
- 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2This antibody detects Human IL-6 under reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Clone MQ2-13A5 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated clone MQ2-39C3 as the detection antibody.
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Secondary Antibodies Available
Negative Isotype Controls Available