IL-6 antibody | MQ2-13A5

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Rat anti Human Interleukin-6

Product Type
Monoclonal Antibody
Clone
MQ2-13A5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1012001 E F* WB* 0.5 mg
Mouse anti Human interleukin-6 antibody, clone MQ2-13A5 recognizes human interleukin 6, also known as B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor or Interferon beta-2. Interleukin-6 is a 183 amino acid ~24 kDa cytokine with a wide range of biological activities. a potent inducer of the acute phase response, acts as a lymphoid cell growth factor acting on B cells, T cells and hybridoma cells.

Variations in the interleukin-6 gene modulates susceptibility to onset of juvenile systemic rheumatoid arthritis (RASJ) an inflammatory disorder with early onset and featuring lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis (Fishman et al. 1998).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Borate buffered saline
Preservative Stabilisers
None present
Immunogen
COS-7-expressed recombinant human IL-6
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from the date of despatch.

More Information

UniProt
P05231 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0001781 neutrophil apoptosis
GO:0002384 hepatic immune response
GO:0002446 neutrophil mediated immunity
GO:0002548 monocyte chemotaxis
GO:0002675 positive regulation of acute inflammatory response
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0006959 humoral immune response
GO:0008083 growth factor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0050829 defense response to Gram-negative bacterium
GO:0008285 negative regulation of cell proliferation
GO:0010574 regulation of vascular endothelial growth factor production
GO:0010888 negative regulation of lipid storage
GO:0045727 positive regulation of translation
GO:0030168 platelet activation
GO:0031018 endocrine pancreas development
GO:0031175 neuron projection development
GO:0032494 response to peptidoglycan
GO:0032722 positive regulation of chemokine production
GO:0032755 positive regulation of interleukin-6 production
GO:0032966 negative regulation of collagen biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042102 positive regulation of T cell proliferation
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0043410 positive regulation of MAPKKK cascade
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045765 regulation of angiogenesis
GO:0045768 positive regulation of anti-apoptosis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050871 positive regulation of B cell activation
GO:0051024 positive regulation of immunoglobulin secretion
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0051607 defense response to virus
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1
Western Blotting 2 1.0 5.0ug/ml
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
  2. 2This antibody detects Human IL-6 under reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
Clone MQ2-13A5 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated clone MQ2-39C3 as the detection antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control MCA6004GA 0.1 mg F

Product Specific References

References for IL-6 antibody

  1. Gilbert, M. et al. (2016) Multiplex single molecule counting (SMC) technology used to generate IL-4, IL-6 and IL-10 reference limits
    Analytical Biochemistry. Mar 24 [Epub ahead of print]