IL-6 antibody | MQ2-13A5
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Rat anti Human Interleukin-6
- Product Type
- Monoclonal Antibody
- Clone
- MQ2-13A5
- Isotype
- IgG1
- Specificity
- IL-6
Antibody Quality and Performance Guarantee
| Rat anti Human interleukin-6 antibody, clone MQ2-13A5 recognizes human interleukin 6, also known as B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor or Interferon beta-2. Interleukin-6 is a 183 amino acid ~24 kDa cytokine with a wide range of biological activities. a potent inducer of the acute phase response, acts as a lymphoid cell growth factor acting on B cells, T cells and hybridoma cells. Variations in the interleukin-6 gene modulates susceptibility to onset of juvenile systemic rheumatoid arthritis (RASJ) an inflammatory disorder with early onset and featuring lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis (Fishman et al. 1998). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- COS-7-expressed recombinant human IL-6
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry 1 | |
||
| Western Blotting 2 | |
1.0 | 5.0 ug/ml |
- 1 Membrane permeabilization is required for this application. We recommend the use of Leucoperm (Product Code BUF09) for this purpose.
- 2This antibody detects Human IL-6 under reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- Clone MQ2-13A5 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA conjugated clone MQ2-39C3 as the detection antibody.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG1 Negative Control | MCA6004GA | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Rat IgG1 Negative Control | ||||||
References for IL-6 antibody
-
Gilbert, M. et al. (2016) Multiplex single molecule counting technology used to generate interleukin 4, interleukin 6, and interleukin 10 reference limits.
Anal Biochem. 503: 11-20.
- RRID
- AB_618532
- UniProt
- P05231
- Entrez Gene
- IL6
- GO Terms
- GO:0001781 neutrophil apoptosis
- GO:0002384 hepatic immune response
- GO:0002446 neutrophil mediated immunity
- GO:0002548 monocyte chemotaxis
- GO:0002675 positive regulation of acute inflammatory response
- GO:0002690 positive regulation of leukocyte chemotaxis
- GO:0006953 acute-phase response
- GO:0005125 cytokine activity
- GO:0005138 interleukin-6 receptor binding
- View More GO Terms
- GO:0005615 extracellular space
- GO:0005896 interleukin-6 receptor complex
- GO:0006959 humoral immune response
- GO:0008083 growth factor activity
- GO:0050830 defense response to Gram-positive bacterium
- GO:0050829 defense response to Gram-negative bacterium
- GO:0008285 negative regulation of cell proliferation
- GO:0010574 regulation of vascular endothelial growth factor production
- GO:0010888 negative regulation of lipid storage
- GO:0045727 positive regulation of translation
- GO:0030168 platelet activation
- GO:0031018 endocrine pancreas development
- GO:0031175 neuron projection development
- GO:0032494 response to peptidoglycan
- GO:0032722 positive regulation of chemokine production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032966 negative regulation of collagen biosynthetic process
- GO:0033138 positive regulation of peptidyl-serine phosphorylation
- GO:0042102 positive regulation of T cell proliferation
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0043066 negative regulation of apoptosis
- GO:0043193 positive regulation of gene-specific transcription
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045079 negative regulation of chemokine biosynthetic process
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045765 regulation of angiogenesis
- GO:0045768 positive regulation of anti-apoptosis
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0050871 positive regulation of B cell activation
- GO:0051024 positive regulation of immunoglobulin secretion
- GO:0051091 positive regulation of transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- GO:0051607 defense response to virus
- GO:0070091 glucagon secretion
- GO:0070102 interleukin-6-mediated signaling pathway
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