IL-4 Receptor antibody | 25463 (49/20)

Mouse anti Human Interleukin-4 Receptor antibody, clone 25463 recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kDa type I single pass transmembrane glycoprotein bearing a single fibronectin type III domain. Interleukin-4 Receptor form a heterodimeric complex with the cytokine receptor common subunit gamma, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

Mouse anti Human Interleukin-4 Receptor antibody, clone 25463 is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND₅₀ was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/250	1/1000
Flow Cytometry		1/10	1/40
Functional Assays
Immunohistology - Paraffin		1/20	1/60
Western Blotting 1		1/125	1/500

1. ¹0100-0462 detects human IL-4 R under non-reducing conditions.