IL-4 Receptor antibody | 25463 (49/20)
Mouse anti Human Interleukin-4 Receptor
- Product Type
- Monoclonal Antibody
- Clone
- 25463 (49/20)
- Isotype
- IgG2a
- Specificity
- IL-4 Receptor
Mouse anti Human Interleukin-4 Receptor antibody, clone 25463 recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kDa type I single pass transmembrane glycoprotein bearing a single fibronectin type III domain. Interleukin-4 Receptor form a heterodimeric complex with the cytokine receptor common subunit gamma, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. Mouse anti Human Interleukin-4 Receptor antibody, clone 25463 is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.5% Trehalose
- Immunogen
- Spodoptera frugiperda lepidopteran ovarian cell line Sf21-derived, recombinant human interleukin 4 receptor, extracellular domain.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/250 | 1/1000 | |
Flow Cytometry | 1/10 | 1/40 | |
Functional Assays | |||
Immunohistology - Paraffin | 1/20 | 1/60 | |
Western Blotting 1 | 1/125 | 1/500 |
- 10100-0462 detects human IL-4 R under non-reducing conditions.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG2a Negative Control |
0100-0462
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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