IL-33 antibody | AbD07786

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Human anti Human Interleukin-33

Product Type
Monoclonal Antibody
Clone
AbD07786
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA140A E 50 µg
HCA140 E 0.1 mg
Human anti Human Interleukin-33 antibody, clone AbD07786 recognizes human Interleukin-33 (IL-33), a cytokine formerly known as NF-HEV (nuclear factor from high endothelial venules).

IL-33 belongs to the IL-1 super family which signals through interaction with the ST2 and Toll-interleukin 1 (IL-1) receptors, and is known to act as an immunoregulator of T helper 2 (Th2) cell cytokine synthesis. IL-33 is abundantly expressed in high endothelial venules and found in tonsils, Peyer's patches and mesenteric lymph nodes.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Pack Size: 50 µg
Reconstitute with 0.5 ml distilled water
Pack Size: 0.1 mg
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human IL33-N1 fusion protein
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
O95760 Related reagents
Entrez Gene
IL33 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0043032 positive regulation of macrophage activation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050729 positive regulation of inflammatory response
GO:0090197 positive regulation of chemokine secretion
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of IL-33 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP1482B as the detection reagent.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Alexa Fluor® 488 MCA1396A488 100 Tests/1ml F
Mouse anti Histidine Tag:Alexa Fluor® 647 MCA1396A647 1 ml F
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti Histidine Tag:FITC MCA1396F 0.1 mg F IF
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Alexa Fluor® 488 MCA2200A488 100 Tests/1ml F*
Mouse anti c-Myc:Alexa Fluor® 647 MCA2200A647 100 Tests/1ml F*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:FITC MCA2200F 0.1 mg F*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti c-Myc:RPE MCA2200PE 100 Tests WB
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
HuCAL Fab-dHLX-MH Negative Control HCA052 0.1 mg C E F P WB

Product Specific References

Further Reading

  1. Joshi, A.D. et al. (2010) Interleukin-33 contributes to both M1 and M2 chemokine marker expression in human macrophages.
    BMC Immunol. 11: 52.