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IL-32 antibody | E03-4C9

Mouse anti Human Interleukin-32

Product Type
Monoclonal Antibody
Clone
E03-4C9
Isotype
IgG1
Specificity
IL-32

Product Code Applications Pack Size List Price Your Price Qty
MCA6212GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human interleukin-32 antibody, clone E03-4C9, recognizes interleukin-32 (IL-32), also known as NK4 and TAIF. IL-32 is a pro-inflammatory cytokine with roles in inflammation and immune regulation, and its expression is strongly regulated by pathogens and pro-inflammatory cytokines (Hong et al. 2017). IL-32 promotes inflammation by upregulating other inflammatory cytokines including TNF-α, IL-1β, IL-6, INF-γ, and IL-8 (Al-Shobaili et al. 2018). Accordingly, a role for IL-32 has been reported in the pathogenesis of chronic inflammatory diseases including IBD, COPD and RA (Damen et al. 2017). IL-32 is highly expressed in several cancer tissues, and plays a crucial role in cancer development through tumor initiation, cell proliferation, and tumor maintenance (Yan et al. 2018).

The purified Mouse anti Human interleukin-32 antibody, clone E03-4C9 (MCA6212GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-32 antibody, clone B01-2A7 (MCA6213B) as the detection antibody.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human interleukin-32 (Cys2-Lys131)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Interleukin-32:Biotin MCA6213B E 0.1 mg loader
List Price Your Price
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Description Mouse anti Human Interleukin-32:Biotin
Recombinant Human INTERLEUKIN-32A PHP301 E 10 µg
List Price Your Price
Description Recombinant Human INTERLEUKIN-32A

UniProt
P24001
Entrez Gene
IL32
GO Terms
GO:0006952 defense response
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response

MCA6212GA

100005152

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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