IL-27 Alpha antibody | H08-5C8

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Mouse anti Human Interleukin 27 Alpha

Product Type
Monoclonal Antibody
Clone
H08-5C8
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5965GA E 0.1 mg
Mouse anti Human IL-27α, clone H08-5C8, recognizes IL-27α, a subunit of IL-27. IL-27 is a 62 kDa member of the Il-6/IL-12 family of long type I cytokines. IL-27 is heterodimeric group 2 receptor composed of Epstein-Barr Virus induced gene 3 (EBI3) and IL-27α. EBI3 is a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23 and IL-27α is a 28 kDa glycoprotein that is related to the p35 of chain IL-12.

IL-27 has broad range of pro- and anti-inflammatory properties, mediating its effects via its gp130-associated cytokine receptor and JAK/STAT signalling cascades. It can alter T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells and has diverse effects on innate immune cells. In vivo, it is reported to play an important role in immune regulation, as mice with defects in IL-27 or its receptor display enhanced immune responses (Batten & Ghilardi 2007).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
NS0-derived recombinant human IL-27
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
Q8NEV9 Related reagents
Entrez Gene
IL27 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0042129 regulation of T cell proliferation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045087 innate immune response
GO:0045523 interleukin-27 receptor binding
GO:0045625 regulation of T-helper 1 cell differentiation
GO:0050688 regulation of defense response to virus
Regulatory
For research purposes only

Applications of IL-27 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Batten, M. & Ghilardi, N. (2007) The biology and therapeutic potential of interleukin 27
    J Mol Med (Berl). 85: 661-72.