IL-27 Alpha antibody | H08-5C8
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Mouse anti Human Interleukin 27 Alpha
- Product Type
- Monoclonal Antibody
- Clone
- H08-5C8
- Isotype
- IgG1
- Specificity
- IL-27 Alpha
Antibody Quality and 
Unrivaled 
| Mouse anti Human IL-27α, clone H08-5C8, recognizes IL-27α, a subunit of IL-27. IL-27 is a 62 kDa member of the Il-6/IL-12 family of long type I cytokines. IL-27 is heterodimeric group 2 receptor composed of Epstein-Barr Virus induced gene 3 (EBI3) and IL-27α. EBI3 is a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23 and IL-27α is a 28 kDa glycoprotein that is related to the p35 of chain IL-12. IL-27 has broad range of pro- and anti-inflammatory properties, mediating its effects via its gp130-associated cytokine receptor and JAK/STAT signalling cascades. It can alter T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells and has diverse effects on innate immune cells. In vivo, it is reported to play an important role in immune regulation, as mice with defects in IL-27 or its receptor display enhanced immune responses (Batten & Ghilardi 2007). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- NS0-derived recombinant human IL-27
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Batten, M. & Ghilardi, N. (2007) The biology and therapeutic potential of interleukin 27
J Mol Med (Berl). 85: 661-72.
- Synonyms
- IL-27 Alpha
- IL27P28
- UniProt
- Q8NEV9
- Entrez Gene
- IL27
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0042129 regulation of T cell proliferation
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045087 innate immune response
- GO:0045523 interleukin-27 receptor binding
- GO:0045625 regulation of T-helper 1 cell differentiation
- GO:0050688 regulation of defense response to virus
- View More GO Terms
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