IL-27 Alpha antibody | H08-5C8

Mouse anti Human Interleukin 27 Alpha

Product Type
Monoclonal Antibody
Clone
H08-5C8
Isotype
IgG1
Specificity
IL-27 Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA5965GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader
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Mouse anti Human IL-27α, clone H08-5C8, recognizes IL-27α, a subunit of IL-27. IL-27 is a 62 kDa member of the Il-6/IL-12 family of long type I cytokines. IL-27 is heterodimeric group 2 receptor composed of Epstein-Barr Virus induced gene 3 (EBI3) and IL-27α. EBI3 is a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23 and IL-27α is a 28 kDa glycoprotein that is related to the p35 of chain IL-12.

IL-27 has broad range of pro- and anti-inflammatory properties, mediating its effects via its gp130-associated cytokine receptor and JAK/STAT signalling cascades. It can alter T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells and has diverse effects on innate immune cells. In vivo, it is reported to play an important role in immune regulation, as mice with defects in IL-27 or its receptor display enhanced immune responses (Batten & Ghilardi 2007).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
NS0-derived recombinant human IL-27
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Further Reading

  1. Batten, M. & Ghilardi, N. (2007) The biology and therapeutic potential of interleukin 27
    J Mol Med (Berl). 85: 661-72.

Synonyms
IL-27 Alpha
IL27P28
UniProt
Q8NEV9
Entrez Gene
IL27
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0042129 regulation of T cell proliferation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045087 innate immune response
GO:0045523 interleukin-27 receptor binding
GO:0045625 regulation of T-helper 1 cell differentiation
GO:0050688 regulation of defense response to virus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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