IL-1RA antibody

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Mouse anti Interleukin-1RA

Mouse anti-human IL-1RA antibody recognizes interleukin-1 receptor antagonist protein, also known as IL-1 inhibitor, IL-1RN (IL-1F3) or intracellular IL-1 receptor antagonist type II. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00500
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human interleukin-1RA antibody recognizes the interleukin-1 receptor antagonist protein, also known as IL1 inhibitor, intracellular interleukin-1 receptor antagonist or type II interleukin-1 receptor antagonist.

The protein encoded by IL1RN gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. IL1RN and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of IL1RN is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human interleukin-1RA antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti interleukin-1RA detects a band of approximately 18 kDa in A431 cell lysate
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to amino acids 26-177 of human interleukin-1RA (IL-1RA)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P18510 Related reagents
Entrez Gene
IL1RN Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005125 cytokine activity
GO:0005152 interleukin-1 receptor antagonist activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005813 centrosome
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0051384 response to glucocorticoid stimulus
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of IL-1RA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
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