IL-1RA antibody

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Anti Interleukin-1RA Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with IL-1RA antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 1 second exposure. Arrow points to IL-1RA (molecular weight 18 kDa).

Mouse anti Interleukin-1RA

Mouse anti-human IL-1RA antibody recognizes interleukin-1 receptor antagonist protein, also known as IL-1 inhibitor, IL-1RN (IL-1F3) or intracellular IL-1 receptor antagonist type II. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00500 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human interleukin-1RA antibody recognizes the interleukin-1 receptor antagonist protein, also known as IL1 inhibitor, intracellular interleukin-1 receptor antagonist or type II interleukin-1 receptor antagonist.

The protein encoded by IL1RN gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. IL1RN and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of IL1RN is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human interleukin-1RA antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti interleukin-1RA detects a band of approximately 18 kDa in A431 cell lysate
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: E. coli derived recombinant protein corresponding to amino acids 26-177 of human interleukin-1RA (IL-1RA)
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P18510 Related reagents
Entrez Gene: IL1RN Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005125 cytokine activity; GO:0005152 interleukin-1 receptor antagonist activity; GO:0005615 extracellular space; GO:0005634 nucleus; GO:0005813 centrosome; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0051384 response to glucocorticoid stimulus
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of IL-1RA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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