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IL-13 antibody | B-P6

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Anti Human Interleukin-13 Antibody, clone B-P6 thumbnail image 1
Anti Human Interleukin-13 Antibody, clone B-P6 gallery image 1
Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated anti Human IL-13 (MCA2791F). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IL-13 (MCA2791F). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human Interleukin-13:FITC

Product Type
Monoclonal Antibody
Clone
B-P6
Isotype
IgG1
Specificity
IL-13

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Product Code Applications Pack Size List Price Your Price Qty
MCA2791F
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Mouse anti Human CD13 antibody, clone B-P6 recognizes human interleukin-13 (IL-13), a 12.5 kDa immunoregulatory cytokine produced primarily by activated T helper type 2 (Th2) cells, and also by mast cells and NK cells.

IL-13 induces the stimulation of B cell proliferation, antibody production and plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E (IgE) and eosinophils (Wills-Karp et al. 1998).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from ascites
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinant human IL-13.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Further Reading

  1. Wills-Karp, M. et al. (1998) Interleukin-13: central mediator of allergic asthma.
    Science. 282 (5397): 2258-61.

RRID
AB_2124187
UniProt
P35225
Entrez Gene
IL13
GO Terms
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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