IL-13 antibody | B-P6

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Anti Human Interleukin-13 Antibody, clone B-P6 gallery image 1 — Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated anti Human IL-13 (MCA2791F). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IL-13 (MCA2791F). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer

Mouse anti Human Interleukin-13:FITC

Product Type: Monoclonal Antibody
Clone: B-P6
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2791F	F*			100 Tests

Mouse anti Human CD13 antibody, clone B-P6 recognizes human interleukin-13 (IL-13), a 12.5 kDa immunoregulatory cytokine produced primarily by activated T helper type 2 (Th2) cells, and also by mast cells and NK cells.

IL-13 induces the stimulation of B cell proliferation, antibody production and plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E (IgE) and eosinophils (Wills-Karp et al. 1998).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by ion exchange chromatography
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Recombinant human IL-13.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P35225 Related reagents
Entrez Gene: IL13 Related reagents
GO Terms: GO:0007165 signal transduction; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0005625 soluble fraction; GO:0006928 cellular component movement; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0007267 cell-cell signaling
Regulatory: For research purposes only

Applications of IL-13 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹

¹Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F