IL-11 antibody | C01-1C2

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Mouse anti Human Interleukin-11

Product Type
Monoclonal Antibody
Clone
C01-1C2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5964GA E 0.1 mg
Mouse anti Human interleukin-11, clone C01-1C2, recognizes human interleukin-11 (IL-11), a 178 amino acid non-glycosylated pleiotropic cytokine, also known as AGIF. IL-11 is a 19 kDa member of the IL-6 of cytokines. It signals through a high affinity receptor complex, made up of the IL-11 receptor alpha chain (IL-11Ralpha) and gp130 which triggers homo- or hetero-dimerization of gp130. Although gp130 and its dimer partners possess no kinase activity, the dimerization of gp130 leads to activation of tyrosine kinases and modulation of transcriptional activity.

IL-11 is an important regulator in the haematopoiesis of erythroid, myeloid and megakaryocyte progenitor cells and is expressed in a variety of tissues including the lung, bone, thymus and connective tissue. Expression of IL-11 in endometrial stromal cells is vital for normal murine embryo development, and implicated in decidualization during human pregnancy. IL-11 is a functionally diverse signalling molecule with roles in megakaryopoiesis (Dams-Kozlowska et al. 2013), bone growth (Sims et al. 2005) and T cell proliferation (Curti et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to aa 23-125 of human IL-11
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P20809 Related reagents
Entrez Gene
IL11 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005142 interleukin-11 receptor binding
GO:0005615 extracellular space
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0030168 platelet activation
GO:0030183 B cell differentiation
GO:0030219 megakaryocyte differentiation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0043410 positive regulation of MAPKKK cascade
GO:0045444 fat cell differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046888 negative regulation of hormone secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
Regulatory
For research purposes only

Applications of IL-11 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Curti, A. et al. (2002) Interleukin-11 induces proliferation of human T-cells and its activity is associated with downregulation of p27(kip1)
    Haematologica 87: 373-80.
  2. Dams-kozlowska, H. et al. (2013) Designer cytokine hyper interleukin 11 (H11) is a megakaryopoietic factor.
    Int J Med Sci. 10 (9): 1157-65.
  3. Sims, N.A. et al. (2005) Interleukin-11 receptor signaling is required for normal bone remodeling
    Journal of Bone and Mineral Research 20: 1093–102.