IKK Epsilon antibody

100% Secure


Mouse anti IKK Epsilon

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00517
Datasheet
WB
SDS
100 µl loader

Mouse anti Human IKK epsilon antibody recognizes the inhibitor of nuclear factor kappa-B kinase subunit epsilon, also known as I-kappa-B kinase epsilon, IKK-epsilon, or inducible I kappa-B kinase.

IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al. 2009).

Mouse anti Human IKK epsilon antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti IKK epsilon detects a band of approximately 80 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
A partial recombinant human IKK epsilon protein (aa2 - 203)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q14164
Entrez Gene
IKBKE
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004704 NF-kappaB-inducing kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0008063 Toll signaling pathway
GO:0008384 IkappaB kinase activity
GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
GO:0010008 endosome membrane
GO:0016605 PML body
GO:0032480 negative regulation of type I interferon production
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of IKK Epsilon antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml