IKK Beta antibody

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Anti IKK ΒETA Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti IKK ΒETA Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with IKK beta antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 20 second exposure. Arrow points to IKK beta (molecular weight 99 kDa).

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Mouse anti IKK ΒETA

Product Type: PrecisionAb Monoclonal
Isotype: IgG2b
Format: Purified
Specificity: IKK Beta

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VMA00575 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human IKK beta antibody recognizes the inhibitor of nuclear factor kappa-B kinase subunit beta, also known as I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK2, IKK-beta or nuclear factor NF-kappa-B inhibitor kinase beta.

The protein encoded by IKBKB gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for IKBKB (provided by RefSeq, Sep 2011).

Mouse anti Human IKK beta antibody detects a band of 99 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti IKK Beta detects a band of approximately 99 kDa in MCF7 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution: HEPES buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen: E. coli-derived recombinant human IKK beta fragment
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: O14920
Entrez Gene: IKBKB
GO Terms: GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0006468 protein phosphorylation; GO:0006916 anti-apoptosis; GO:0008063 Toll signaling pathway; GO:0008384 IkappaB kinase activity; GO:0042802 identical protein binding; View More GO Terms; GO:0009898 internal side of plasma membrane; GO:0016563 transcription activator activity; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0035631 CD40 receptor complex; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0045087 innate immune response; GO:0045121 membrane raft; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050852 T cell receptor signaling pathway; GO:0051092 positive regulation of NF-kappaB transcription factor activity