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IKK antibody

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Western Blot Validated
Anti IKK Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti IKK Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti IKK Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of Hela, MCF-7 and Jurkat whole cell lysates probed with Mouse anti IKK (VMA00667) at 1/1000 dilution with 22 second exposure. Arrow points to IKK.
Anti IKK Antibody (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates probed with IKK antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 37 second exposure. Arrow points to IKK (molecular weight 85 & 87 kDa).

Mouse anti IKK

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00667
Datasheet
WB
SDS
100 µl loader

Mouse anti Human IKK antibody recognizes inhibitor of nuclear factor (NF) kappa-B kinase subunit alpha, also known as I-kappa-B kinase 1, IKK-a kinase, IkB kinase alpha subunit or nuclear factor NFkappaB inhibitor kinase alpha.

CHUK encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor (provided by RefSeq, Jul 2008).

Mouse anti Human IKK antibody detects bands of 85 and 87 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti IKK antibody detects bands of approximately 85 and 87 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Full length recombinant IKK

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
O15111
Entrez Gene
CHUK
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0007252 I-kappaB phosphorylation
GO:0008063 Toll signaling pathway
GO:0008384 IkappaB kinase activity
GO:0042802 identical protein binding
GO:0009898 internal side of plasma membrane
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0035631 CD40 receptor complex
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of IKK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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