IKK antibody

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Anti IKK Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti IKK Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of Hela, MCF-7 and Jurkat whole cell lysates probed with Mouse anti IKK (**VMA00667**) at 1/1000 dilution with 22 second exposure. Arrow points to IKK.

Anti IKK Antibody (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates probed with IKK antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 37 second exposure. Arrow points to IKK (molecular weight 85 & 87 kDa).

Mouse anti IKK

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00667 Datasheet	WB SDS	100 µl	Log in		Get Quote

Mouse anti Human IKK antibody recognizes inhibitor of nuclear factor (NF) kappa-B kinase subunit alpha, also known as I-kappa-B kinase 1, IKK-a kinase, IkB kinase alpha subunit or nuclear factor NFkappaB inhibitor kinase alpha.

CHUK encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor (provided by RefSeq, Jul 2008).

Mouse anti Human IKK antibody detects bands of 85 and 87 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Mouse anti IKK antibody detects bands of approximately 85 and 87 kDa in K562 cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Full length recombinant IKK

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Guarantee: 12 months from date of despatch

More Information

UniProt: O15111
Entrez Gene: CHUK
GO Terms: GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0007252 I-kappaB phosphorylation; GO:0008063 Toll signaling pathway; GO:0008384 IkappaB kinase activity; GO:0042802 identical protein binding; GO:0009898 internal side of plasma membrane; GO:0048015 phosphatidylinositol-mediated signaling; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0035631 CD40 receptor complex; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050852 T cell receptor signaling pathway; GO:0051092 positive regulation of NF-kappaB transcription factor activity
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of IKK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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