Ikaros antibody | 4E9

Mouse anti Human Ikaros

Product Type
Monoclonal Antibody
Clone
4E9
Isotype
IgG1
Specificity
Ikaros

Product Code Applications Pack Size List Price Your Price Qty
MCA6137
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * IC IP WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human Ikaros, clone 4E9 recognizes Ikaros also known as Ikaros family zinc finger protein 1 (IFZF1) a DNA binding protein. It is expressed by hematopoietic cells, lymphoid cells and some subpopulations of myeloid cells. Ikaros is required for lymphocyte development and mediates B and T cell differentiation. It is thought that Ikaros regulates these processes by altering transcription of its target genes through chromatin remodeling of DNA promoter regions (Gowda et al. 2017).

To regulate transcription, Ikaros forms homo- or heterodimers with other Ikaros family members such as Helios, Aiolos, Eos or Pegasus. This dimerization forms a complex which is capable of binding DNA and initiating its transcriptional activity (Merkenschlager 2010).

This clone 4E9 has been used in immunocytochemistry experiments to examine Ikaros expression in cells incubated with cholesterol to examine how the transcription factor modulates cholesterol uptake (Loeper et al. 2008).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant human Ikaros (C-terminal)
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunocytochemistry
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
IFZF1
UniProt
Q13422
Entrez Gene
IKZF1
GO Terms
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0007049 cell cycle
GO:0007498 mesoderm development
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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