Ikaros antibody | 4E9
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F ResetMouse anti Human Ikaros
- Product Type
- Monoclonal Antibody
- Clone
- 4E9
- Isotype
- IgG1
- Specificity
- Ikaros
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| Mouse anti Human Ikaros, clone 4E9 recognizes Ikaros also known as Ikaros family zinc finger protein 1 (IFZF1) a DNA binding protein. It is expressed by hematopoietic cells, lymphoid cells and some subpopulations of myeloid cells. Ikaros is required for lymphocyte development and mediates B and T cell differentiation. It is thought that Ikaros regulates these processes by altering transcription of its target genes through chromatin remodeling of DNA promoter regions (Gowda et al. 2017). To regulate transcription, Ikaros forms homo- or heterodimers with other Ikaros family members such as Helios, Aiolos, Eos or Pegasus. This dimerization forms a complex which is capable of binding DNA and initiating its transcriptional activity (Merkenschlager 2010). This clone 4E9 has been used in immunocytochemistry experiments to examine Ikaros expression in cells incubated with cholesterol to examine how the transcription factor modulates cholesterol uptake (Loeper et al. 2008). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant human Ikaros (C-terminal)
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
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| Immunocytochemistry | |
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| Immunoprecipitation | |
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| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
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| Erythrolyse Red Blood Cell Lysing Buffer (10x) | BUF04B | F | 100 Test/20ml | Log in | |||
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| Description | Erythrolyse Red Blood Cell Lysing Buffer (10x) | ||||||
- Synonyms
- IFZF1
- UniProt
- Q13422
- Entrez Gene
- IKZF1
- GO Terms
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0007049 cell cycle
- GO:0007498 mesoderm development
- GO:0008270 zinc ion binding
- GO:0016568 chromatin modification
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