IgM anti NP antibody | THM

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Chimaeric Human IgM anti NP

Product Type
Monoclonal Antibody
Clone
THM
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA446S E 2 ml
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgM antibody specific for NP has been produced.

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).
Fusion Partners
Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgM anti NP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IgM anti NP antibody

  1. Neuberger, M.S. et al. (1984) Recombinant antibodies possessing novel effector functions.
    Nature 312: 604-608.
  2. Neuberger, M.S. (1985) Making Novel Antibodies by expressing transfected immunoglobulin genes.
    TIBS 347-349.
  3. Neuberger, M.S. et al. (1985) A hapten-specific chimaeric IgE antibody with Human physiological effector function.
    Nature 314: 268-270.
  4. Neuberger, M.S., Williams, G.T. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors.
    Philos. Trans. R. Soc. Lond. Ser. A: Hath. Phys. Sci. 317: 425-432.