IgG3 antibody | HP6050
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Mouse anti Human IgG3 antibody, clone HP6050 recognizes the hinge region of the heavy chain of human IgG3.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared from ascites
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 50% Glycerol
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Pool of 5-6 individually purified Human IgG3 myeloma proteins.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 - Ag14/X myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01860
- Entrez Gene
- IGHG3
- GO Terms
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IgG3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/4000 | 1/10,000 | |
| Immunohistology - Frozen | |||
| Western Blotting | |||
| ELISA | |||
| Flow Cytometry | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG3 antibody
-
Jefferis, R. et al. (1985) Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.
Immunol Lett. 10 (3-4): 223-52. -
Jefferis, R. & Kumararatne, D.S. (1990) Selective IgG subclass deficiency: quantification and clinical relevance.
Clin Exp Immunol. 81 (3): 357-67. -
Mutapi, F. et al. (2002) The effect of treatment on the age-antibody relationship in children infected with Schistosoma mansoni and Schistosoma haematobium.
Mem Inst Oswaldo Cruz. 97 Suppl 1: 173-80.
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