IgG2 antibody | HP6200

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Mouse anti Human IgG2 F(ab')2

Product Type
Monoclonal Antibody
Clone
HP6200
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1011G C* E R WB 0.2 mg
Mouse anti Human IgG2 F(ab')2 antibody, clone HP6200 recognizes human IgG2, binding to an epitope in the F(ab')2 region incorporating the proline residue (AA 234).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Semi-purified normal human IgG2.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.<

This product should be stored undiluted.br />
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01859 Related reagents
Entrez Gene
IGHG2 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1
Immunohistology - Paraffin
Radioimmunoassays
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Application Based External Images

ELISA

Product Specific References

References for IgG2 antibody

  1. Jefferis, R. et al. (1992) Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study.
    Immunol Lett. 31 (2): 143-68.
  2. Harada, S. et al. (1989) Evaluation of production and characterization of monoclonal antibodies to human IgG of four subclasses.
    Microbiol Immunol. 33 (7): 579-92.
  3. Harada, S. et al. (1991) Identification of epitopes recognized by a panel of six anti-human IgG2 monoclonal antibodies.
    J Immunol Methods. 141 (1): 89-96.
  4. Harada, S. et al. (1992) Hinge region of human IgG2 protein: conformational studies with monoclonal antibodies.
    Mol Immunol. 29 (2): 145-9.
  5. Findlow, H. et al. (2006) Immunoglobulin G subclass response to a meningococcal quadrivalent polysaccharide-diphtheria toxoid conjugate vaccine.
    Clin Vaccine Immunol. 13: 507-10.