IgG2 antibody | HP6014

Mouse anti Human IgG2

Product Type
Monoclonal Antibody
Clone
HP6014
Isotype
IgG1
Specificity
IgG2
Region
(Fab)

Product Code Applications Pack Size List Price Your Price Qty
MCA515G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human IgG2 antibody, clone HP6014 reacts with human IgG2 at an epitope localised to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by haemagglutination assays.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified Human IgG2.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)

References for IgG2 antibody

  1. Jefferis, R. et al. (1985) Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.
    Immunol Lett. 10 (3-4): 223-52.
  2. Hussain, R. et al. (1986) Use of monoclonal antibodies to quantify subclasses of human IgG. I. Development of two-site immunoenzymometric assays for total IgG subclass determinations.
    J Immunol Methods. 93 (1): 89-96.
  3. Jefferis, R. & Kumararatne, D.S. (1990) Selective IgG subclass deficiency: quantification and clinical relevance.
    Clin Exp Immunol. 81 (3): 357-67.
  4. Mutapi, F. et al. (2002) The effect of treatment on the age-antibody relationship in children infected with Schistosoma mansoni and Schistosoma haematobium.
    Mem Inst Oswaldo Cruz. 97 Suppl 1: 173-80.
  5. Ranaivoson, F.M. et al. (2019) A Proteomic Screen of Neuronal Cell-Surface Molecules Reveals IgLONs as Structurally Conserved Interaction Modules at the Synapse.
    Structure. Mar 23 [Epub ahead of print].

RRID
AB_321812
UniProt
P01859
Entrez Gene
IGHG2
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products