IgG2 anti NP antibody | JW183

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Chimaeric Human IgG2 anti NP

Product Type
Monoclonal Antibody
Clone
JW183
Isotype
IgG2
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA334B E 0.5 mg
Chimaeric Human HUMAN IgG2 anti NP antibody, clone JW183combines an immunoglobulin heavy chain produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. The J558L cell-line itself secretes a lambda light chain but no heavy chain. Thus a chimaeric human IgG2 antibody specific for NP has been produced

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgG2 anti NP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Application Based External Images

Immunoassay

Product Specific References

References for IgG2 anti NP antibody

  1. Neuberger, M.S. et al. (1984) Recombinant antibodies possessing novel effector functions.
    Nature 312: 604-608.
  2. Neuberger, M.S. et al. (1985) A hapten-specific chimaeric IgE antibody with human physiological effector function.
    Nature 314: 268-270.
  3. Neuberger, M.S. et al. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors.
    Trans. R. Soc. Lond. A317: 425-432.
  4. Wines, B.D. et al. (2003) Soluble FcgammaRIIa inhibits rheumatoid factor binding to immune complexes.
    Immunology. 109: 246-54.