IgG2 anti NP antibody | JW183
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Chimaeric Human HUMAN IgG2 anti NP antibody, clone JW183combines an immunoglobulin heavy chain produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. The J558L cell-line itself secretes a lambda light chain but no heavy chain. Thus a chimaeric human IgG2 antibody specific for NP has been produced
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- 1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of IgG2 anti NP antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG2 anti NP antibody
-
Neuberger, M.S. et al. (1984) Recombinant antibodies possessing novel effector functions.
Nature 312: 604-608. -
Neuberger, M.S. et al. (1985) A hapten-specific chimaeric IgE antibody with human physiological effector function.
Nature 314: 268-270. -
Neuberger, M.S. et al. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors.
Trans. R. Soc. Lond. A317: 425-432. -
Wines, B.D. et al. (2003) Soluble FcgammaRIIa inhibits rheumatoid factor binding to immune complexes.
Immunology. 109: 246-54.
