Mouse anti Human IgG (CH3 Domain) antibody, clone A57H reacts with the CH3 domain of human IgG
- Target Species
- Product Form
- Purified IgM - liquid
- Purified IgM prepared by thiosorb chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- pFc' from purified protein of Heavy Chain Disease.
- Approx. Protein Concentrations
- IgM concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0 mouse myeloma cell line.
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
complement activation, classical pathway
innate immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgG antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for IgG antibody
Nelson, P.N. et al. (1990) Evaluation of monoclonal antibodies with putative specificity for human IgG allotypes.
Vox Sang. 59 (3): 190-7.
Nelson, P.N. et al. (1994) Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants.
Immunol Invest. 23 (1): 39-45.
Komatsuda, A. et al. (2008) Monoclonal immunoglobulin deposition disease associated with membranous features.
Nephrol Dial Transplant. 23 (12): 3888-94.
Thomson, C.A. et al. (2011) Somatic Diversity in CDR3 Loops Allows Single V-Genes To Encode Innate Immunological Memories for Multiple Pathogens.
J Immunol. 186: 2291-8.
Komatsuda, A. et al. (2013) Proliferative glomerulonephritis with discrete deposition of monoclonal immunoglobulin γ1 C(H) 2 heavy chain and κ light chain: A new variant of monoclonal immunoglobulin deposition disease.
Pathol Int. 63: 63-7.
Ruffini, P.A. et al. (2014) Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
J Transl Med. 12: 207.
Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
Mech Ageing Dev. 152: 15-31.
Kato, H. et al. (2015) Rapid Deterioration of the Renal Function Caused by the Coexistence of Intratubular Amyloidosis and Myeloma Cast Nephropathy.
Intern Med. 54 (23): 3023-8.
Grodeland G et al. (2016) Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.
J Immunol. Sep 26. pii: 1600893. [Epub ahead of print]
Baranowska, M. et al. (2015) Targeting of nucleoprotein to chemokine receptors by DNA vaccination results in increased CD8(+)-mediated cross protection against influenza.
Vaccine. 33 (49): 6988-96.
Braathen, R. et al. (2018) The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules
ImmunoHorizons. 2 (1): 38-53.