IgG antibody | A57H

Mouse anti Human IgG (CH3 Domain)

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E H WB 0.2 mg loader
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Mouse anti Human IgG (CH3 Domain) antibody, clone A57H reacts with the CH3 domain of human IgG

Target Species
Product Form
Purified IgM - liquid
Purified IgM prepared by thiosorb chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
pFc' from purified protein of Heavy Chain Disease.
Approx. Protein Concentrations
IgM concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0 mouse myeloma cell line.
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgG antibody

  1. Nelson, P.N. et al. (1990) Evaluation of monoclonal antibodies with putative specificity for human IgG allotypes.
    Vox Sang. 59 (3): 190-7.
  2. Nelson, P.N. et al. (1994) Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants.
    Immunol Invest. 23 (1): 39-45.
  3. Komatsuda, A. et al. (2008) Monoclonal immunoglobulin deposition disease associated with membranous features.
    Nephrol Dial Transplant. 23 (12): 3888-94.
  4. Thomson, C.A. et al. (2011) Somatic Diversity in CDR3 Loops Allows Single V-Genes To Encode Innate Immunological Memories for Multiple Pathogens.
    J Immunol. 186: 2291-8.
  5. Komatsuda, A. et al. (2013) Proliferative glomerulonephritis with discrete deposition of monoclonal immunoglobulin γ1 C(H) 2 heavy chain and κ light chain: A new variant of monoclonal immunoglobulin deposition disease.
    Pathol Int. 63: 63-7.
  6. Ruffini, P.A. et al. (2014) Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
    J Transl Med. 12: 207.
  7. Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
    Mech Ageing Dev. 152: 15-31.
  8. Kato, H. et al. (2015) Rapid Deterioration of the Renal Function Caused by the Coexistence of Intratubular Amyloidosis and Myeloma Cast Nephropathy.
    Intern Med. 54 (23): 3023-8.
  9. View The Latest Product References
  10. Grodeland, G. et al. (2016) Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.
    J Immunol. 197 (9): 3575-85.
  11. Baranowska, M. et al. (2015) Targeting of nucleoprotein to chemokine receptors by DNA vaccination results in increased CD8(+)-mediated cross protection against influenza.
    Vaccine. 33 (49): 6988-96.
  12. Braathen, R. et al. (2018) The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules
    ImmunoHorizons. 2 (1): 38-53.
  13. Grodeland, G. et al. (2020) Targeting of HA to chemokine receptors induces strong and cross-reactive T cell responses after DNA vaccination in pigs.
    Vaccine. 38 (6): 1280-5.

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response


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