IgG antibody | A57H
Mouse anti Human IgG (CH3 domain) antibody, clone A57H used as a coating antibody in a capture ELISA for vaccibodies containing the IgG (CH3 domain).
Image caption:
Characterization of fusion vaccines used in this study. (A) Schematic structure of a homodimeric vaccine fusion protein (Vaccibody, VB). Targeting, dimerization and antigenic units are indicated, as are the different moieties expressed in the various units. MHC class II targeted (I-Ed-specific) and non-targeted (NIP-specific) constructs are denoted (FvI-EFvPn)2 and (FvNIPFvPn)2, respectively, whereas LD78β VB are denoted (LD78βFvPn)2. where Pn indicates patient number. (B) Detection of VB proteins in supernatants of transfected HEK 293E cells in an ELISA specific for hγCH3. (C) Binding of MHC class II-targeted VB to I-Ed+ A20 cells. (D) LD78β VB displays dose-dependent chemotactic activity on lymphocytic Esb/MP cells. Recombinant LD78β was used as positive control.
From: Ruffini PA, Os A, Dolcetti R, Tjønnfjord GE, Munthe LA, Bogen B.
Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
J Transl Med. 2014 Jul 25;12:207.
Mouse anti Human IgG (CH3 Domain) antibody, clone A57H reacts with the CH3 domain of human IgG
Product Details
- Target Species
- Human
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by thiosorb chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- pFc' from purified protein of Heavy Chain Disease.
- Approx. Protein Concentrations
- IgM concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01857 Related reagents
- P01859 Related reagents
- P01834 Related reagents
- P01861 Related reagents
- P01860 Related reagents
- Entrez Gene
- IGKC Related reagents
- IGHG1 Related reagents
- IGHG2 Related reagents
- IGHG3 Related reagents
- IGHG4 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Haemagglutination | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | 0.1 mg | F | ||
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | 1 ml | C E P WB | ||
| Goat anti Mouse IgM:RPE | STAR86PE | 0.5 ml | F | ||
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | 1 mg | C E WB | ||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | 1 mg | E |
Product Specific References
References for IgG antibody
-
Nelson, P.N. et al. (1990) Evaluation of monoclonal antibodies with putative specificity for human IgG allotypes.
Vox Sang. 59 (3): 190-7. -
Nelson, P.N. et al. (1994) Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants.
Immunol Invest. 23 (1): 39-45. -
Komatsuda, A. et al. (2008) Monoclonal immunoglobulin deposition disease associated with membranous features.
Nephrol Dial Transplant. 23 (12): 3888-94. -
Thomson, C.A. et al. (2011) Somatic Diversity in CDR3 Loops Allows Single V-Genes To Encode Innate Immunological Memories for Multiple Pathogens.
J Immunol. 186: 2291-8. -
Komatsuda, A. et al. (2013) Proliferative glomerulonephritis with discrete deposition of monoclonal immunoglobulin γ1 C(H) 2 heavy chain and κ light chain: A new variant of monoclonal immunoglobulin deposition disease.
Pathol Int. 63: 63-7. -
Ruffini, P.A. et al. (2014) Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
J Transl Med. 12: 207. -
Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
Mech Ageing Dev. 152: 15-31. -
Kato, H. et al. (2015) Rapid Deterioration of the Renal Function Caused by the Coexistence of Intratubular Amyloidosis and Myeloma Cast Nephropathy.
Intern Med. 54 (23): 3023-8. -
Grodeland G et al. (2016) Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.
J Immunol. Sep 26. pii: 1600893. [Epub ahead of print] -
Baranowska, M. et al. (2015) Targeting of nucleoprotein to chemokine receptors by DNA vaccination results in increased CD8(+)-mediated cross protection against influenza.
Vaccine. 33 (49): 6988-96. -
Braathen, R. et al. (2018) The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules
ImmunoHorizons. 2 (1): 38-53.
