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IgG antibody | A57H

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Anti Human IgG (CH3 Domain) Antibody, clone A57H gallery image 1
Published customer image:
Mouse anti Human IgG (CH3 domain) antibody, clone A57H used as a coating antibody in a capture ELISA for vaccibodies containing the IgG (CH3 domain).
Image caption:
Characterization of fusion vaccines used in this study. (A) Schematic structure of a homodimeric vaccine fusion protein (Vaccibody, VB). Targeting, dimerization and antigenic units are indicated, as are the different moieties expressed in the various units. MHC class II targeted (I-Ed-specific) and non-targeted (NIP-specific) constructs are denoted (FvI-EFvPn)2 and (FvNIPFvPn)2, respectively, whereas LD78β VB are denoted (LD78βFvPn)2. where Pn indicates patient number. (B) Detection of VB proteins in supernatants of transfected HEK 293E cells in an ELISA specific for hγCH3. (C) Binding of MHC class II-targeted VB to I-Ed+ A20 cells. (D) LD78β VB displays dose-dependent chemotactic activity on lymphocytic Esb/MP cells. Recombinant LD78β was used as positive control.

From: Ruffini PA, Os A, Dolcetti R, Tjønnfjord GE, Munthe LA, Bogen B.
Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
J Transl Med. 2014 Jul 25;12:207.

Mouse anti Human IgG (CH3 Domain)

Product Type
Monoclonal Antibody
Clone
A57H
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
MCA878G
Datasheet
E H WB
SDS
0.2 mg loader

Mouse anti Human IgG (CH3 Domain) antibody, clone A57H reacts with the CH3 domain of human IgG

Product Details

Target Species
Human
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by thiosorb chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
pFc' from purified protein of Heavy Chain Disease.
Approx. Protein Concentrations
IgM concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01857
P01859
P01834
P01861
P01860
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Haemagglutination
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Product Specific References

References for IgG antibody

  1. Nelson, P.N. et al. (1990) Evaluation of monoclonal antibodies with putative specificity for human IgG allotypes.
    Vox Sang. 59 (3): 190-7.
  2. Nelson, P.N. et al. (1994) Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants.
    Immunol Invest. 23 (1): 39-45.
  3. Komatsuda, A. et al. (2008) Monoclonal immunoglobulin deposition disease associated with membranous features.
    Nephrol Dial Transplant. 23 (12): 3888-94.
  4. Thomson, C.A. et al. (2011) Somatic Diversity in CDR3 Loops Allows Single V-Genes To Encode Innate Immunological Memories for Multiple Pathogens.
    J Immunol. 186: 2291-8.
  5. Komatsuda, A. et al. (2013) Proliferative glomerulonephritis with discrete deposition of monoclonal immunoglobulin γ1 C(H) 2 heavy chain and κ light chain: A new variant of monoclonal immunoglobulin deposition disease.
    Pathol Int. 63: 63-7.
  6. Ruffini, P.A. et al. (2014) Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
    J Transl Med. 12: 207.
  7. Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
    Mech Ageing Dev. 152: 15-31.
  8. Kato, H. et al. (2015) Rapid Deterioration of the Renal Function Caused by the Coexistence of Intratubular Amyloidosis and Myeloma Cast Nephropathy.
    Intern Med. 54 (23): 3023-8.
  9. Grodeland G et al. (2016) Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.
    J Immunol. Sep 26. pii: 1600893. [Epub ahead of print]
  10. Baranowska, M. et al. (2015) Targeting of nucleoprotein to chemokine receptors by DNA vaccination results in increased CD8(+)-mediated cross protection against influenza.
    Vaccine. 33 (49): 6988-96.
  11. Braathen, R. et al. (2018) The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules
    ImmunoHorizons. 2 (1): 38-53.

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