IgG antibody | A57H

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Mouse anti Human IgG (CH3 Domain)

Product Type
Monoclonal Antibody
Clone
A57H
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA878G E H WB 0.2 mg
Mouse anti Human IgG (CH3 Domain) antibody, clone A57H reacts with the CH3 domain of human IgG

Product Details

Target Species
Human
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by thiosorb chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
pFc' from purified protein of Heavy Chain Disease.
Approx. Protein Concentrations
IgM concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01857 Related reagents
P01859 Related reagents
P01834 Related reagents
P01861 Related reagents
P01860 Related reagents
Entrez Gene
IGKC Related reagents
IGHG1 Related reagents
IGHG2 Related reagents
IGHG3 Related reagents
IGHG4 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Haemagglutination
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Product Specific References

References for IgG antibody

  1. Nelson, P.N. et al. (1990) Evaluation of monoclonal antibodies with putative specificity for human IgG allotypes.
    Vox Sang. 59 (3): 190-7.
  2. Nelson, P.N. et al. (1994) Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants.
    Immunol Invest. 23 (1): 39-45.
  3. Komatsuda, A. et al. (2008) Monoclonal immunoglobulin deposition disease associated with membranous features.
    Nephrol Dial Transplant. 23 (12): 3888-94.
  4. Thomson, C.A. et al. (2011) Somatic Diversity in CDR3 Loops Allows Single V-Genes To Encode Innate Immunological Memories for Multiple Pathogens.
    J Immunol. 186: 2291-8.
  5. Komatsuda, A. et al. (2013) Proliferative glomerulonephritis with discrete deposition of monoclonal immunoglobulin γ1 C(H) 2 heavy chain and κ light chain: A new variant of monoclonal immunoglobulin deposition disease.
    Pathol Int. 63: 63-7.
  6. Ruffini, P.A. et al. (2014) Targeted DNA vaccines eliciting crossreactive anti-idiotypic antibody responses against human B cell malignancies in mice.
    J Transl Med. 12: 207.
  7. Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
    Mech Ageing Dev. 152: 15-31.
  8. Kato, H. et al. (2015) Rapid Deterioration of the Renal Function Caused by the Coexistence of Intratubular Amyloidosis and Myeloma Cast Nephropathy.
    Intern Med. 54 (23): 3023-8.
  9. Grodeland G et al. (2016) Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.
    J Immunol. Sep 26. pii: 1600893. [Epub ahead of print]
  10. Baranowska, M. et al. (2015) Targeting of nucleoprotein to chemokine receptors by DNA vaccination results in increased CD8(+)-mediated cross protection against influenza.
    Vaccine. 33 (49): 6988-96.
  11. Braathen, R. et al. (2018) The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules
    ImmunoHorizons. 2 (1): 38-53.